biostudies-literatureUnknownConoan Nieves NENIGMS NIH HHSThe Class II NAD⁺ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg²⁺.bioRxiv : the preprint server for biology2024.11.10.622884https://www.ebi.ac.uk/biostudies/studies/S-EPMC11601409biostudies-literatureLigand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry.PMC11601409R35 GM147229Conoan Nieves NEWidom JRfalseLigand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry.The Class II NAD⁺ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg²⁺.2024-01-01T00:00:00Z2024 Nov2026-04-07T14:53:23.667Z2025-04-03T23:56:17.119ZS-EPMC116014093960570310.1101/2024.11.10.622884