{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Berger KD"],"funding":["NIEHS NIH HHS","NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["All tRNAs undergo a series of chemical modifications to fold and function correctly. In mammals, the C32 nucleotide in the anticodon loop of tRNA-Arg-CCU and UCU is methylated to form 3-methylcytosine (m3C). Deficiency of m3C in arginine tRNAs has been linked to human neurodevelopmental disorders, indicating a critical biological role for m3C modification. However, the structural repercussions of m3C modification are not well understood. Here, we examine the structural effects of m3C32 modification on the anticodon stem loop (ASL) of human tRNA-Arg-UCU-4-1, a unique tRNA with enriched expression in the central nervous system. Optical melting experiments demonstrate that m3C modification can locally disrupt nearby base pairing within the ASL while simultaneously stabilizing the ASL electros"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.11.18.624017"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11601484"],"repository":["biostudies-literature"],"pubmed_title":["Structural impact of 3-methylcytosine modification on the anticodon stem of a neuronally-enriched arginine tRNA."],"pmcid":["PMC11601484"],"funding_grant_id":["R01 GM129325","R35 GM145283","P30 ES001247","P41 GM111135","S10 OD012254","R01 GM143145"],"pubmed_authors":["Fu D","Puthenpeedikakkal AMK","Mathews DH","Berger KD"],"additional_accession":[]},"is_claimable":false,"name":"Structural impact of 3-methylcytosine modification on the anticodon stem of a neuronally-enriched arginine tRNA.","description":"All tRNAs undergo a series of chemical modifications to fold and function correctly. In mammals, the C32 nucleotide in the anticodon loop of tRNA-Arg-CCU and UCU is methylated to form 3-methylcytosine (m3C). Deficiency of m3C in arginine tRNAs has been linked to human neurodevelopmental disorders, indicating a critical biological role for m3C modification. However, the structural repercussions of m3C modification are not well understood. Here, we examine the structural effects of m3C32 modification on the anticodon stem loop (ASL) of human tRNA-Arg-UCU-4-1, a unique tRNA with enriched expression in the central nervous system. Optical melting experiments demonstrate that m3C modification can locally disrupt nearby base pairing within the ASL while simultaneously stabilizing the ASL electros","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-19T03:17:31.465Z","creation":"2025-04-03T23:55:52.614Z"},"accession":"S-EPMC11601484","cross_references":{"pubmed":["39605410"],"doi":["10.1101/2024.11.18.624017"]}}