biostudies-literatureHuang QNational Key R&D Program of ChinaNational Natural Science Founding of China grantsShanghai Frontiers Science Center of Cellular Homeostasis and Human Diseases, the Innovation Program of Shanghai Municipal Education CommissionShanghai Municipal Commission of Health and Family PlanningInnovative research team of high-level local universities in Shanghaie178079https://www.ebi.ac.uk/biostudies/studies/S-EPMC11601948biostudies-literatureUnknown134(23)Ku70, a DNA repair protein, binds to the damaged DNA ends and orchestrates the recruitment of other proteins to facilitate repair of DNA double-strand breaks. Besides its essential role in DNA repair, several studies have highlighted nonclassical functions of Ku70 in cellular processes. However, its function in immune homeostasis and antitumor immunity remains unknown. Here, we discovered a marked association between elevated Ku70 expression and unfavorable prognosis in lung adenocarcinoma, focusing specifically on increased Ku70 levels in tumor-infiltrated Tregs. Using a lung-colonizing tumor model in mice with Treg-specific Ku70 deficiency, we demonstrated that deletion of Ku70 in Tregs led to a stronger antitumor response and slower tumor growth due to impaired immune-suppressive capaciThe Journal of clinical investigationNonclassical action of Ku70 promotes Treg-suppressive function through a FOXP3-dependent mechanism in lung adenocarcinoma.PMC1160194882241222822718292023ZD0501605202040117SHSMU-ZDCX2021060121140902900321300412023ZD0507404319611330112019YFA09006102Zhou QZhang JLi DWang CDu YHuang QYe YDai XLiu XZhang WChen ZSong SLi BTian NCheng HDai SMLiang RfalseNonclassical action of Ku70 promotes Treg-suppressive function through a FOXP3-dependent mechanism in lung adenocarcinoma.Ku70, a DNA repair protein, binds to the damaged DNA ends and orchestrates the recruitment of other proteins to facilitate repair of DNA double-strand breaks. Besides its essential role in DNA repair, several studies have highlighted nonclassical functions of Ku70 in cellular processes. However, its function in immune homeostasis and antitumor immunity remains unknown. Here, we discovered a marked association between elevated Ku70 expression and unfavorable prognosis in lung adenocarcinoma, focusing specifically on increased Ku70 levels in tumor-infiltrated Tregs. Using a lung-colonizing tumor model in mice with Treg-specific Ku70 deficiency, we demonstrated that deletion of Ku70 in Tregs led to a stronger antitumor response and slower tumor growth due to impaired immune-suppressive capaci2024-01-01T00:00:00Z2024 Oct2026-06-02T23:10:25.825Z2025-04-06T01:57:19.25ZS-EPMC116019483944649310.1172/JCI178079