{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Arad M"],"funding":["Natural Sciences and Engineering Research Council of Canada","University of the Fraser Valley","BC Knowledge Development Fund","Canada Foundation for Innovation","Genome British Columbia"],"pagination":["18880-18889"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11603402"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(47)"],"pubmed_abstract":["A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes, <i>in situ</i> pipette tips, and automated robotic liquid handling. Factorial experimental design identified a 1 h, room temperature, pepsin-trypsin dual-enzyme digestion as optimal for sequence coverage and protein group identification, comparable to a gold-standard overnight proteomic protocol. This method promises significant advancements in proteomic analysis by enhancing reusability, speed, throughput, convenience, and cost-effectiveness, without hindering digestion efficienc"],"journal":["Analytical chemistry"],"pubmed_title":["Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels."],"pmcid":["PMC11603402"],"funding_grant_id":["374PRO"],"pubmed_authors":["Nestman Z","Jereb D","Fordwour O","Balagtas R","Frey C","Shi Y","Ku K","Hare R","Sidhu A","Moon KM","Ghafourifar G","Foster LJ","Arad M"],"additional_accession":[]},"is_claimable":false,"name":"Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels.","description":"A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes, <i>in situ</i> pipette tips, and automated robotic liquid handling. Factorial experimental design identified a 1 h, room temperature, pepsin-trypsin dual-enzyme digestion as optimal for sequence coverage and protein group identification, comparable to a gold-standard overnight proteomic protocol. This method promises significant advancements in proteomic analysis by enhancing reusability, speed, throughput, convenience, and cost-effectiveness, without hindering digestion efficienc","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-01T16:23:15.824Z","creation":"2025-04-04T02:33:27.245Z"},"accession":"S-EPMC11603402","cross_references":{"pubmed":["39528415"],"doi":["10.1021/acs.analchem.4c04846"]}}