biostudies-literatureZou HMNatural Science Foundation of Guizhou ProvinceNational Natural Science Foundation of ChinaScience and Technology Foundation of Guizhou Provincial Health CommissionNational Natural Science Foundation of China’s (NSFC) Cultivation Projecte001349https://www.ebi.ac.uk/biostudies/studies/S-EPMC11603702biostudies-literatureUnknown11(2)<h4>Objective</h4>The current study aims to elucidate the critical function of hepatocyte nuclear factor 1-beta (HNF1-β) in lupus nephritis (LN) by investigating its modulation of the Derlin-1/valosin-containing protein (VCP)/VCP-interacting membrane selenoprotein (VIMP) complex, endoplasmic reticulum (ER) stress and podocyte apoptosis.<h4>Methods</h4>In vitro and in vivo models of LN were established using glomerular podocytes treated with LN serum and MRL/lpr mice, respectively. The expression levels of HNF1-β were analysed in kidney tissues from patients with LN and MRL/lpr mice. To assess the effects of HNF1-β inhibition, an adeno-associated virus vector carrying HNF1-β short hairpin was administered to MRL/lpr mice. In vitro, glomerular podocytes were transfected with HNF1-β small intLupus science & medicineHNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.PMC1160370282360147gyfynsfc[2022]-53QianKeHeJiChu-ZK[2022] General 378gzwkj2023-20982460148QianKeHeJiChu-ZK[2022] General 409Zhang FLiu JZou HMXie YShi MJLiu MLuo YQYu JAn XMXu LFChen PLGuo BRuan YYfalseHNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.<h4>Objective</h4>The current study aims to elucidate the critical function of hepatocyte nuclear factor 1-beta (HNF1-β) in lupus nephritis (LN) by investigating its modulation of the Derlin-1/valosin-containing protein (VCP)/VCP-interacting membrane selenoprotein (VIMP) complex, endoplasmic reticulum (ER) stress and podocyte apoptosis.<h4>Methods</h4>In vitro and in vivo models of LN were established using glomerular podocytes treated with LN serum and MRL/lpr mice, respectively. The expression levels of HNF1-β were analysed in kidney tissues from patients with LN and MRL/lpr mice. To assess the effects of HNF1-β inhibition, an adeno-associated virus vector carrying HNF1-β short hairpin was administered to MRL/lpr mice. In vitro, glomerular podocytes were transfected with HNF1-β small int2024-01-01T00:00:00Z2024 Nov2025-04-19T04:22:25.35Z2025-04-19T04:22:25.35ZS-EPMC116037023960881510.1136/lupus-2024-001349