biostudies-literatureRojo-Carrillo JJCentro de Investigación Biomédica en red de Enfermedades Raras, SpainFundación Española de Trombosis y HemostasiaInstituto de Salud Carlos IIIMinisterio de Ciencia e Innovación1148https://www.ebi.ac.uk/biostudies/studies/S-EPMC11603882biostudies-literatureUnknown25(1)<h4>Background</h4>Protein-coding genes have been considered the functional part of the genome, although they represent only 2% of the genome. In contrast, more than 90% of the genome produces non-coding RNA (ncRNA), including antisense (AS) genes, a type of long non-coding genes (encoding transcripts > 200 nucleotides) located on the opposite strand of coding genes. Therefore, antisense RNA (asRNA) can be complementary to the counterpart sense RNA, supporting a regulatory role with potential pathogenic consequences, as their deregulation has been associated with cardiovascular disease, cancer, and diabetes.<h4>Results</h4>We performed an in-depth review of AS genes in Ensembl and evaluated the expression of AS genes in human liver by third-generation RNA sequencing methods. Currently, 165BMC genomicsLandscape of antisense genes in the human genome and identification of new human hepatic antisense RNAs by long-read sequencing.PMC11603882RYC2021-031000-IPI21/00174Predoctoral fellowshipCB15/00055PMP21/00052Padilla JLlamas-Lopez MLozano MLGarrido-Rodriguez Pde la Morena-Barrio MERojo-Carrillo JJRamos-Molina BCifuentes-Riquelme Rde la Morena-Barrio BCorral JfalseLandscape of antisense genes in the human genome and identification of new human hepatic antisense RNAs by long-read sequencing.<h4>Background</h4>Protein-coding genes have been considered the functional part of the genome, although they represent only 2% of the genome. In contrast, more than 90% of the genome produces non-coding RNA (ncRNA), including antisense (AS) genes, a type of long non-coding genes (encoding transcripts > 200 nucleotides) located on the opposite strand of coding genes. Therefore, antisense RNA (asRNA) can be complementary to the counterpart sense RNA, supporting a regulatory role with potential pathogenic consequences, as their deregulation has been associated with cardiovascular disease, cancer, and diabetes.<h4>Results</h4>We performed an in-depth review of AS genes in Ensembl and evaluated the expression of AS genes in human liver by third-generation RNA sequencing methods. Currently, 1652024-01-01T00:00:00Z2024 Nov2026-06-02T05:02:31.991Z2025-04-04T00:46:20.158ZS-EPMC116038823960485110.1186/s12864-024-11017-3