biostudies-literatureApaolaza PSLeona M. and Harry B. Helmsley Charitable TrustHorizon 2020 Framework ProgrammeHelmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)JDRFBreakthrough T1D Canada (formerly JDRF Canada)Canadian Institutes of Health ResearchCIHR2771-2785https://www.ebi.ac.uk/biostudies/studies/S-EPMC11604696biostudies-literatureUnknown67(12)<h4>Aims/hypothesis</h4>Islet prohormone-processing enzymes convert peptide hormone precursors to mature hormones. Defective beta cell prohormone processing and the release of incompletely processed peptide hormones are observed prior to the onset of diabetes, yet molecular mechanisms underlying impaired prohormone processing during the development of diabetes remains largely unknown. Previous studies have shown that prohormone convertase 1/3 (PC1/3) protein and mRNA expression levels are reduced in whole islets from donors with type 1 diabetes, although whether PC1/3-mediated prohormone processing in alpha and beta cells is disrupted in type 1 diabetes remained to be explored. Herein, we aimed to analyse the expression of PC1/3 in islets from non-diabetic donors, autoantibody-positive donDiabetologiaQuantitative analysis of islet prohormone convertase 1/3 expression in human pancreas donors with diabetes.PMC11604696No 115797No 945268AWD-019767 JDFC 20213-APF-2022-1141-A-N5-CDA-2020-949-A-N3-COE-2022-1103-M-BCIHR PJT-153156Apaolaza PSRodriguez-Calvo TBoulassel SChen YCLurz YGrewal KVerchere CBfalseQuantitative analysis of islet prohormone convertase 1/3 expression in human pancreas donors with diabetes.<h4>Aims/hypothesis</h4>Islet prohormone-processing enzymes convert peptide hormone precursors to mature hormones. Defective beta cell prohormone processing and the release of incompletely processed peptide hormones are observed prior to the onset of diabetes, yet molecular mechanisms underlying impaired prohormone processing during the development of diabetes remains largely unknown. Previous studies have shown that prohormone convertase 1/3 (PC1/3) protein and mRNA expression levels are reduced in whole islets from donors with type 1 diabetes, although whether PC1/3-mediated prohormone processing in alpha and beta cells is disrupted in type 1 diabetes remained to be explored. Herein, we aimed to analyse the expression of PC1/3 in islets from non-diabetic donors, autoantibody-positive don2024-01-01T00:00:00Z2024 Dec2026-06-02T10:41:26.911Z2025-04-04T01:24:12.625ZS-EPMC116046963940484410.1007/s00125-024-06275-5