biostudies-literatureLiu YFundamental Research Funds for the Central UniversitiesResearch Project of Jinan Microecological Biomedicine Shandong LaboratoryShandong Provincial Laboratory ProjectCAMS Innovation Fund for Medical SciencesZhejiang Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of China506https://www.ebi.ac.uk/biostudies/studies/S-EPMC11606014biostudies-literatureUnknown24(1)<h4>Background</h4>Klebsiella pneumoniae has emerged as a significant pathogen that causes community and hospital infections due to its high resistance rate and transmissibility. Klebsiella pneumoniae complex is classified into three phylogroups: Klebsiella pneumoniae (KpI), Klebsiella quasipneumoniae (KpII), and Klebsiella variicola (KpIII) in classical taxonomy, but KpII and KpIII are infrequently isolated clinically. Although pathogenic KpII has been reported worldwide, the understanding of this pathogen remains limited.<h4>Methods</h4>Whole-genome sequencing (WGS) of K. quasipneumoniae subsp. similipneumoniae ACESH00366hy was performed. Plasmid characterization was demonstrated using S1-PFGE, Southern blotting, and conjugation assays. Antimicrobial susceptibility testing was performed BMC microbiologyGenomic and phenotypic characterization of ST2012 clinical Klebsiella quasipneumoniae subsp. similipneumoniae harboring bla_NDM-1 in China.PMC11606014LHDMZ22H1900022020YFE0204300SYS20220282072314JNL-2022011B2019-I2M-5-0452022ZFJH003Liu XLiu YQian JChen MZhang XZheng BLiu RXu HfalseGenomic and phenotypic characterization of ST2012 clinical Klebsiella quasipneumoniae subsp. similipneumoniae harboring bla_NDM-1 in China.<h4>Background</h4>Klebsiella pneumoniae has emerged as a significant pathogen that causes community and hospital infections due to its high resistance rate and transmissibility. Klebsiella pneumoniae complex is classified into three phylogroups: Klebsiella pneumoniae (KpI), Klebsiella quasipneumoniae (KpII), and Klebsiella variicola (KpIII) in classical taxonomy, but KpII and KpIII are infrequently isolated clinically. Although pathogenic KpII has been reported worldwide, the understanding of this pathogen remains limited.<h4>Methods</h4>Whole-genome sequencing (WGS) of K. quasipneumoniae subsp. similipneumoniae ACESH00366hy was performed. Plasmid characterization was demonstrated using S1-PFGE, Southern blotting, and conjugation assays. Antimicrobial susceptibility testing was performed 2024-01-01T00:00:00Z2024 Nov2026-06-03T01:03:15.005Z2025-04-04T01:58:12.726ZS-EPMC116060143961414810.1186/s12866-024-03637-2