{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(2)"],"submitter":["Yao L"],"pubmed_abstract":["Biosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity. In this study, <i>gadB</i> <sub>ΔC11</sub> of <i>Lactiplantibacillus plantarum</i> and <i>gadC</i> <sub>ΔC41</sub> of <i>Escherichia coli</i> were inserted into the designed double promoter (P <i><sub>T7lac</sub></i> and P <i><sub>BAD</sub></i> ) expression system. Then, <i>E. coli</i> Lemo21(DE3) was chosen as the host to minimize the toxic effects of GadC<sub>ΔC41</sub> overexpression. Furthermore, a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3) cells as bioc"],"journal":["Engineering microbiology"],"pagination":["100077"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11610993"],"repository":["biostudies-literature"],"pubmed_title":["High-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in &lt;i&gt;Escherichia coli&lt;/i&gt;."],"pmcid":["PMC11610993"],"pubmed_authors":["Yao L","Mei L","Hu S","Cao H","Huang J","Zhao W","Lyu C","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"High-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in &lt;i&gt;Escherichia coli&lt;/i&gt;.","description":"Biosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity. In this study, <i>gadB</i> <sub>ΔC11</sub> of <i>Lactiplantibacillus plantarum</i> and <i>gadC</i> <sub>ΔC41</sub> of <i>Escherichia coli</i> were inserted into the designed double promoter (P <i><sub>T7lac</sub></i> and P <i><sub>BAD</sub></i> ) expression system. Then, <i>E. coli</i> Lemo21(DE3) was chosen as the host to minimize the toxic effects of GadC<sub>ΔC41</sub> overexpression. Furthermore, a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3) cells as bioc","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-27T02:10:55.243Z","creation":"2025-04-06T18:31:29.107Z"},"accession":"S-EPMC11610993","cross_references":{"pubmed":["39629248"],"doi":["10.1016/j.engmic.2023.100077"]}}