biostudies-literatureUnknown3(2)Yao LBiosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity. In this study, gadB ΔC11 of Lactiplantibacillus plantarum and gadC ΔC41 of Escherichia coli were inserted into the designed double promoter (P T7lac and P BAD ) expression system. Then, E. coli Lemo21(DE3) was chosen as the host to minimize the toxic effects of GadCΔC41 overexpression. Furthermore, a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3) cells as biocEngineering microbiology100077https://www.ebi.ac.uk/biostudies/studies/S-EPMC11610993biostudies-literatureHigh-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in Escherichia coli.PMC11610993Yao LMei LHu SCao HHuang JZhao WLyu CWang YfalseHigh-level production of γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in Escherichia coli.Biosynthesis of the functional factor γ-aminobutyric acid (GABA) in bacteria involves two key proteins an intracellular glutamate decarboxylase (GadB) and a membrane-bound antiporter (GadC). Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity. In this study, gadB ΔC11 of Lactiplantibacillus plantarum and gadC ΔC41 of Escherichia coli were inserted into the designed double promoter (P T7lac and P BAD ) expression system. Then, E. coli Lemo21(DE3) was chosen as the host to minimize the toxic effects of GadCΔC41 overexpression. Furthermore, a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3) cells as bioc2023-01-01T00:00:00Z2023 Jun2025-04-27T02:10:55.243Z2025-04-06T18:31:29.107ZS-EPMC116109933962924810.1016/j.engmic.2023.100077