biostudies-literatureZhu HScience and Technology Plan Project of Taizhou739https://www.ebi.ac.uk/biostudies/studies/S-EPMC11615178biostudies-literatureUnknown15(1)Disulfidptosis is a newfound programmed cell death (PCD) mode characterized by disulfide stress. Nevertheless, the characteristics and functional significance of disulfidptosis-related genes in head and neck squamous cell carcinoma (HNSCC) are still largely unknown. In this study, several computer-aided bioinformatic analyses were performed. The Nonnegative Matrix Factorization (NMF) method classified The Cancer Genome Atlas (TCGA) patients into two clusters according to the expression of disulfidptosis-related genes. The relative compositions of cells in the tumor microenvironment (TME), mutant landscape, lasso regression analysis, and predicted clinical outcome were performed by analyzing bulk RNA-sequencing data. Besides, single-cell sequencing data (scRNA) was analyzed by Seurat, CopyKDiscover oncologyThe characteristics and functional significance of disulfidptosis-related genes in head and neck squamous cell carcinoma.PMC1161517823ywa20Hu CZhao CZhang ZZhu HXu XfalseThe characteristics and functional significance of disulfidptosis-related genes in head and neck squamous cell carcinoma.Disulfidptosis is a newfound programmed cell death (PCD) mode characterized by disulfide stress. Nevertheless, the characteristics and functional significance of disulfidptosis-related genes in head and neck squamous cell carcinoma (HNSCC) are still largely unknown. In this study, several computer-aided bioinformatic analyses were performed. The Nonnegative Matrix Factorization (NMF) method classified The Cancer Genome Atlas (TCGA) patients into two clusters according to the expression of disulfidptosis-related genes. The relative compositions of cells in the tumor microenvironment (TME), mutant landscape, lasso regression analysis, and predicted clinical outcome were performed by analyzing bulk RNA-sequencing data. Besides, single-cell sequencing data (scRNA) was analyzed by Seurat, CopyK2024-01-01T00:00:00Z2024 Dec2025-04-04T00:45:51.21Z2025-04-04T00:45:51.21ZS-EPMC116151783962566010.1007/s12672-024-01629-2