biostudies-literatureUnknown52(5)Dinsa Guta RInstitute of Planning and Evaluation for Technology in Food, Agriculture and Forestry<i>Pseudoperonospora cubensis</i> is a significant phytopathogen causing downy mildew disease in cucurbit crops. Understanding the molecular mechanisms underlying the interaction between <i>P. cubensis</i> and its host is essential for developing effective disease management strategies. RxLR effectors, secreted by pathogens, play a crucial role in modulating host immunity. In this study, we sequenced the genome of the <i>P. cubensis</i> isolate CNU-OTH and identified RxLR effector genes using bioinformatics tools. A total of 45 RxLR effector genes were identified from the genome of <i>P. cubensis</i>. Cloning and functional characterization of these effectors were performed through transient expression assays in <i>Nicotiana benthamiana</i> leaves. Subcellular localization of selected effeMycobiology306-316https://www.ebi.ac.uk/biostudies/studies/S-EPMC11619035biostudies-literatureIdentification of <i>Pseudoperonospora cubensis</i> RxLR Effector Genes <i>via</i> Genome Sequencing.PMC11619035Jeong ISemunyana MMin JArif SKim SHDinsa Guta ROh SKfalseIdentification of <i>Pseudoperonospora cubensis</i> RxLR Effector Genes <i>via</i> Genome Sequencing.<i>Pseudoperonospora cubensis</i> is a significant phytopathogen causing downy mildew disease in cucurbit crops. Understanding the molecular mechanisms underlying the interaction between <i>P. cubensis</i> and its host is essential for developing effective disease management strategies. RxLR effectors, secreted by pathogens, play a crucial role in modulating host immunity. In this study, we sequenced the genome of the <i>P. cubensis</i> isolate CNU-OTH and identified RxLR effector genes using bioinformatics tools. A total of 45 RxLR effector genes were identified from the genome of <i>P. cubensis</i>. Cloning and functional characterization of these effectors were performed through transient expression assays in <i>Nicotiana benthamiana</i> leaves. Subcellular localization of selected effe2024-01-01T00:00:00Z20242026-05-26T20:12:47.56Z2025-04-19T14:37:42.27ZS-EPMC116190353964914510.1080/12298093.2024.2408064