{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Patino M"],"funding":["National Institute of Neurological Disorders and Stroke","NEI NIH HHS","National Eye Institute","National Cancer Institute","NINDS NIH HHS","National Institutes of Health","National Institute of General Medical Sciences","NIH HHS","NIGMS NIH HHS"],"pagination":["3851-3866.e4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11624072"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112(23)"],"pubmed_abstract":["Complex neocortical functions rely on networks of diverse excitatory and inhibitory neurons. While local connectivity rules between major neuronal subclasses have been established, the specificity of connections at the level of transcriptomic subtypes remains unclear. We introduce single transcriptome assisted rabies tracing (START), a method combining monosynaptic rabies tracing and single-nuclei RNA sequencing to identify transcriptomic cell types, providing inputs to defined neuron populations. We employ START to transcriptomically characterize inhibitory neurons providing monosynaptic input to 5 different layer-specific excitatory cortical neuron populations in mouse primary visual cortex (V1). At the subclass level, we observe results consistent with findings from prior studies that resolve neuronal subclasses using antibody staining, transgenic mouse lines, and morphological reconstruction. With improved neuronal subtype granularity achieved with START, we demonstrate transcriptomic subtype specificity of inhibitory inputs to various excitatory neuron subclasses. These results establish local connectivity rules at the resolution of transcriptomic inhibitory cell types."],"journal":["Neuron"],"pubmed_title":["Transcriptomic cell-type specificity of local cortical circuits."],"pmcid":["PMC11624072"],"funding_grant_id":["T32 GM007198","P30 014195","R01 EY022577","S10-OD023689","R34 NS116885","S10 OD023689"],"pubmed_authors":["Rossa MA","Patne NS","Patino M","Lagos WN","Callaway EM"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic cell-type specificity of local cortical circuits.","description":"Complex neocortical functions rely on networks of diverse excitatory and inhibitory neurons. While local connectivity rules between major neuronal subclasses have been established, the specificity of connections at the level of transcriptomic subtypes remains unclear. We introduce single transcriptome assisted rabies tracing (START), a method combining monosynaptic rabies tracing and single-nuclei RNA sequencing to identify transcriptomic cell types, providing inputs to defined neuron populations. We employ START to transcriptomically characterize inhibitory neurons providing monosynaptic input to 5 different layer-specific excitatory cortical neuron populations in mouse primary visual cortex (V1). At the subclass level, we observe results consistent with findings from prior studies that resolve neuronal subclasses using antibody staining, transgenic mouse lines, and morphological reconstruction. With improved neuronal subtype granularity achieved with START, we demonstrate transcriptomic subtype specificity of inhibitory inputs to various excitatory neuron subclasses. These results establish local connectivity rules at the resolution of transcriptomic inhibitory cell types.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-07-15T06:15:58.565Z","creation":"2026-06-30T03:22:57.783Z"},"accession":"S-EPMC11624072","cross_references":{"pubmed":["39353431"],"doi":["10.1016/j.neuron.2024.09.003"]}}