{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(4)"],"submitter":["Xiang H"],"pubmed_abstract":["Tobacco (<i>Nicotiana tabacum</i>) plants synthesize the psychoactive pyridine alkaloid nicotine, which has sparked growing interest in reducing nicotine levels through genome editing aiming at inactivating key biosynthetic genes. Although stable transformation-mediated genome editing is effective in tobacco, its polyploid nature complicates the complete knockout of genes and the segregation of transgenes from edited plants. In this study, we developed a non-transgenic genome editing method in tobacco by delivering the CRISPR/Cas machinery via an engineered negative-strand RNA rhabdovirus vector, followed by the regeneration of mutant plants through tissue culture. Using this method, we targeted six <i>berberine bridge enzyme-like protein</i> (<i>BBL</i>) family genes for mutagenesis, whic"],"journal":["aBIOTECH"],"pagination":["449-464"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11624166"],"repository":["biostudies-literature"],"pubmed_title":["Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation of &lt;i&gt;berberine bridge enzyme-like&lt;/i&gt; mutants with reduced nicotine content."],"pmcid":["PMC11624166"],"pubmed_authors":["Gao Q","Jiang J","Li Z","Wang S","Huang H","Mi Q","Xiang H","Ni S","Chen B","Zeng W","Kong W"],"additional_accession":[]},"is_claimable":false,"name":"Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation of &lt;i&gt;berberine bridge enzyme-like&lt;/i&gt; mutants with reduced nicotine content.","description":"Tobacco (<i>Nicotiana tabacum</i>) plants synthesize the psychoactive pyridine alkaloid nicotine, which has sparked growing interest in reducing nicotine levels through genome editing aiming at inactivating key biosynthetic genes. Although stable transformation-mediated genome editing is effective in tobacco, its polyploid nature complicates the complete knockout of genes and the segregation of transgenes from edited plants. In this study, we developed a non-transgenic genome editing method in tobacco by delivering the CRISPR/Cas machinery via an engineered negative-strand RNA rhabdovirus vector, followed by the regeneration of mutant plants through tissue culture. Using this method, we targeted six <i>berberine bridge enzyme-like protein</i> (<i>BBL</i>) family genes for mutagenesis, whic","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T23:31:36.119Z","creation":"2025-04-04T23:31:36.119Z"},"accession":"S-EPMC11624166","cross_references":{"pubmed":["39650142"],"doi":["10.1007/s42994-024-00188-y"]}}