biostudies-literatureUnknown15Kang Q<i>Ophiocordyceps sinensis</i>, an entomopathogenic fungus, infects larvae from the Lepidoptera: Hepialidae family, forming the valuable Chinese cordyceps. Mycoviruses are widespread across major lineages of filamentous fungi, oomycetes, and yeasts and have the potential to influence fungal biology and ecology. This study aimed to detect mycovirus within <i>O. sinensis</i> by isolating double-stranded RNA from six stains for transcriptomic sequencing and analyzing publicly available transcriptome data from 13 <i>O. sinensis</i> representative samples. Our analysis revealed 13 mycoviruses, with nine reported for the first time in <i>O. sinensis</i>. These mycoviruses are distributed across five families-<i>Partitiviridae</i>, <i>Mitoviridae</i>, <i>Narnaviridae</i>, <i>Botourmiaviridae</i>,Frontiers in microbiology1493365https://www.ebi.ac.uk/biostudies/studies/S-EPMC11625762biostudies-literatureUnveiling mycoviral diversity in <i>Ophiocordyceps sinensis</i> through transcriptome analyses.PMC11625762Qin QLi XKang QZhang JMeng QZhang HChen FWang HDong CfalseUnveiling mycoviral diversity in <i>Ophiocordyceps sinensis</i> through transcriptome analyses.<i>Ophiocordyceps sinensis</i>, an entomopathogenic fungus, infects larvae from the Lepidoptera: Hepialidae family, forming the valuable Chinese cordyceps. Mycoviruses are widespread across major lineages of filamentous fungi, oomycetes, and yeasts and have the potential to influence fungal biology and ecology. This study aimed to detect mycovirus within <i>O. sinensis</i> by isolating double-stranded RNA from six stains for transcriptomic sequencing and analyzing publicly available transcriptome data from 13 <i>O. sinensis</i> representative samples. Our analysis revealed 13 mycoviruses, with nine reported for the first time in <i>O. sinensis</i>. These mycoviruses are distributed across five families-<i>Partitiviridae</i>, <i>Mitoviridae</i>, <i>Narnaviridae</i>, <i>Botourmiaviridae</i>,2024-01-01T00:00:00Z20242026-06-03T05:32:51.556Z2025-04-04T01:57:38.041ZS-EPMC116257623965467310.3389/fmicb.2024.1493365