biostudies-literatureJi ZSpecial Research Fund for Clinical Studies of Innovative Drugs after Market LaunchTianjin Science and Technology Commission key projectPhD Start-up Foundation of Tianjin Medical University Cancer Institute and HospitalTianjin Key Medical Discipline (Specialty) Construction Projecte010041https://www.ebi.ac.uk/biostudies/studies/S-EPMC11629098biostudies-literatureUnknown12(12)<h4>Background</h4>Perioperative chemotherapy is the standard of care for patients with locally advanced gastric and gastroesophageal junction cancer. Recent evidence demonstrated the addition of programmed cell death protein 1 (PD-1) inhibitors enhanced therapeutic efficacy. However, the mechanisms of response and resistance remain largely undefined. A detailed multiomic investigation is essential to elucidate these mechanisms.<h4>Methods</h4>We performed whole-exome sequencing, whole-transcriptome sequencing, multiplex immunofluorescence and single-cell RNA sequencing on matched pretreatment and post-treatment samples from 30 patients enrolled in an investigator-initiated Phase 2 clinical trial (NCT04908566). All patients received neoadjuvant PD-1 inhibitors in combination with chemotherJournal for immunotherapy of cancerMultiomics reveals tumor microenvironment remodeling in locally advanced gastric and gastroesophageal junction cancer following neoadjuvant immunotherapy and chemotherapy.PMC11629098WKZX2024CX102217B1806TJYXZDXK-009A21JCZDJC00980B2204Li HDeng TMa LLv XBa YSu LLiu RZhang LXin JGe SLiu YZuo DZhang YDing TJi ZWang XfalseMultiomics reveals tumor microenvironment remodeling in locally advanced gastric and gastroesophageal junction cancer following neoadjuvant immunotherapy and chemotherapy.<h4>Background</h4>Perioperative chemotherapy is the standard of care for patients with locally advanced gastric and gastroesophageal junction cancer. Recent evidence demonstrated the addition of programmed cell death protein 1 (PD-1) inhibitors enhanced therapeutic efficacy. However, the mechanisms of response and resistance remain largely undefined. A detailed multiomic investigation is essential to elucidate these mechanisms.<h4>Methods</h4>We performed whole-exome sequencing, whole-transcriptome sequencing, multiplex immunofluorescence and single-cell RNA sequencing on matched pretreatment and post-treatment samples from 30 patients enrolled in an investigator-initiated Phase 2 clinical trial (NCT04908566). All patients received neoadjuvant PD-1 inhibitors in combination with chemother2024-01-01T00:00:00Z2024 Dec2026-06-02T04:38:37.76Z2025-04-19T14:37:35.391ZS-EPMC116290983965355410.1136/jitc-2024-010041