{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(11)"],"submitter":["Ghosh S"],"pubmed_abstract":["Neutrophils are indispensable for defense against pathogens. Injured tissue-infiltrated neutrophils can establish a niche of chronic inflammation and promote degeneration. Studies investigated transcriptome of single-infiltrated neutrophils which could misinterpret molecular states of these post mitotic cells. However, neutrophil proteome characterization has been challenging due to low harvests from affected tissues. Here, we present a workflow to obtain proteome of 1000 murine and human tissue-infiltrated neutrophils. We generated spectral libraries containing ∼6200 mouse and ∼5300 human proteins from circulating neutrophils. 4800 mouse and 3400 human proteins were recovered from 1000 cells with 10<sup>2</sup>-10<sup>8</sup> copies/cell. Neutrophils from stroke-affected mouse brains adap"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["100858"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11630641"],"repository":["biostudies-literature"],"pubmed_title":["Proteomic Characterization of 1000 Human and Murine Neutrophils Freshly Isolated From Blood and Sites of Sterile Inflammation."],"pmcid":["PMC11630641"],"pubmed_authors":["Lange E","Gruneboom A","Richter M","Sickmann A","Heyer R","Stenzel M","Soehnlein O","Beer A","Ghosh S","Tuz AA","Nagel D","Cibir Z","Jung M","Hermann DM","Hasenberg A","Gunzer M","Singh V"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic Characterization of 1000 Human and Murine Neutrophils Freshly Isolated From Blood and Sites of Sterile Inflammation.","description":"Neutrophils are indispensable for defense against pathogens. Injured tissue-infiltrated neutrophils can establish a niche of chronic inflammation and promote degeneration. Studies investigated transcriptome of single-infiltrated neutrophils which could misinterpret molecular states of these post mitotic cells. However, neutrophil proteome characterization has been challenging due to low harvests from affected tissues. Here, we present a workflow to obtain proteome of 1000 murine and human tissue-infiltrated neutrophils. We generated spectral libraries containing ∼6200 mouse and ∼5300 human proteins from circulating neutrophils. 4800 mouse and 3400 human proteins were recovered from 1000 cells with 10<sup>2</sup>-10<sup>8</sup> copies/cell. Neutrophils from stroke-affected mouse brains adap","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-02T04:38:12.765Z","creation":"2025-04-19T14:35:59.206Z"},"accession":"S-EPMC11630641","cross_references":{"pubmed":["39395581"],"doi":["10.1016/j.mcpro.2024.100858"]}}