biostudies-literatureGuo XTaishan Scholarship Young Expert ProgramNatural Science Foundation of Shandong ProvinceNational Natural Science Foundation of ChinaYouth Innovation of Colleges and Universities of Shandong Province of ChinaKey Research and Development Program of Shandong ProvinceNational Key Research and Development Program of Chinae70128https://www.ebi.ac.uk/biostudies/studies/S-EPMC11632201biostudies-literatureUnknown30(12)<h4>Background</h4>The presence of glioma stem cells (GSCs) and the occurrence of mesenchymal phenotype transition contribute to the miserable prognosis of glioblastoma (GBM). Cellular communication network factor 1 (CCN1) is upregulated within various malignancies and associated with cancer development and progression, while the implications of CCN1 in the phenotype transition and tumorigenicity of GSCs remain unclear.<h4>Methods</h4>Data for bioinformatic analysis were obtained from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. A range of primary GBM and GSC cell models were then used to demonstrate the regulatory role of CCN1 via the phenotype validation, tumor sphere formation assays, extreme limiting dilution assays (ELDA), and transwell assays. To sCNS neuroscience & therapeuticsCCN1 Promotes Mesenchymal Phenotype Transition Through Activating NF-κB Signaling Pathway Regulated by S100A8 in Glioma Stem Cells.PMC11632201ZR2023MH0362023YFC24130042022YFC2406404822724132023KJ023tsqn2019091742021ZLGX02Tian FFan YGuo XWang CGao ZXu SGuo SfalseCCN1 Promotes Mesenchymal Phenotype Transition Through Activating NF-κB Signaling Pathway Regulated by S100A8 in Glioma Stem Cells.<h4>Background</h4>The presence of glioma stem cells (GSCs) and the occurrence of mesenchymal phenotype transition contribute to the miserable prognosis of glioblastoma (GBM). Cellular communication network factor 1 (CCN1) is upregulated within various malignancies and associated with cancer development and progression, while the implications of CCN1 in the phenotype transition and tumorigenicity of GSCs remain unclear.<h4>Methods</h4>Data for bioinformatic analysis were obtained from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. A range of primary GBM and GSC cell models were then used to demonstrate the regulatory role of CCN1 via the phenotype validation, tumor sphere formation assays, extreme limiting dilution assays (ELDA), and transwell assays. To s2024-01-01T00:00:00Z2024 Dec2025-04-22T13:09:29.434Z2025-04-06T00:35:46.233ZS-EPMC116322013965923610.1111/cns.70128