biostudies-literatureLiu ZShanghai Science and Technology CommitteeScience and Technology Commission of Shanghai MunicipalityNational Natural Science Foundation of Chinae2407517https://www.ebi.ac.uk/biostudies/studies/S-EPMC11633487biostudies-literatureUnknown11(46)Tumor immune microenvironment is strongly associated with the malignancy behavior of hepatocellular carcinoma (HCC). However, the immune function and regulatory mechanisms of B cells in HCC remain unclear. The expression differences between B cell high- and low-infiltration HCC samples are explored to identify the key regulator. Pre-mRNA processing factor 19 (PRP19) expression is increased in B cell low-infiltrated tissues and negatively correlated with the B cell marker, CD20. Inhibition of PRP19 expression promoted B cell infiltration in tumor tissue and impeded HCC growth. Mechanically, the co-immunoprecipitation (Co-IP) assay revealed that PRP19 interacts with DEAD-box helicase 5 (DDX5), leading to ubiquitination and degradation of the DDX5 protein. The attenuated DDX5 impairs CXCL12 mAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Increased PRP19 in Hepatocyte Impedes B Cell Function to Promote Hepatocarcinogenesis.PMC116334878220386320Y1190810082403314821731228227302781972234Zhang FLiu ZZhang DXue RLin XDong LGuo DTang WZhang SShen XYu XfalseIncreased PRP19 in Hepatocyte Impedes B Cell Function to Promote Hepatocarcinogenesis.Tumor immune microenvironment is strongly associated with the malignancy behavior of hepatocellular carcinoma (HCC). However, the immune function and regulatory mechanisms of B cells in HCC remain unclear. The expression differences between B cell high- and low-infiltration HCC samples are explored to identify the key regulator. Pre-mRNA processing factor 19 (PRP19) expression is increased in B cell low-infiltrated tissues and negatively correlated with the B cell marker, CD20. Inhibition of PRP19 expression promoted B cell infiltration in tumor tissue and impeded HCC growth. Mechanically, the co-immunoprecipitation (Co-IP) assay revealed that PRP19 interacts with DEAD-box helicase 5 (DDX5), leading to ubiquitination and degradation of the DDX5 protein. The attenuated DDX5 impairs CXCL12 m2024-01-01T00:00:00Z2024 Dec2026-06-02T11:17:45.929Z2025-04-06T15:06:52.832ZS-EPMC116334873942206310.1002/advs.202407517