biostudies-literatureZhou ZNational Natural Science Foundation of Distinguished Young ScholarsJiangsu Provincial Key Laboratory of Critical Care MedicineNatural Science Foundation Outstanding Youth Fund of Jiangsu ProvinceNational Natural Science Foundation of ChinaJiangsu provincial doctors of entrepreneurship and innovation2030-Major Project of the Ministry of Science and Technology of ChinaFundamental Research Funds for the Central Universities grantNatural Science Foundation of Jiangsu Provincee2404391https://www.ebi.ac.uk/biostudies/studies/S-EPMC11633488biostudies-literatureUnknown11(46)Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision-tuning of the function of astrocytic processes is garnering increasing attention because of its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca²⁺) transients in astrocytic processes are pivotal for the functional regulation of these processes. However, the understanding of the alterations and regulatory mechanism of microdomain Ca²⁺ transients during stroke remains limited. In the present study, a fast high-resolution, miniaturized two-photon microscopy is used to show that the levels of astrocytic microdomain Ca²⁺ transients are significantly reduced in the peri-infarct area of awake ischemic stroke mice. ThisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Membrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice.PMC1163348882230115(2020)302938200373382473903822739142242024RCB0026BK202003582242022R40059819035912021ZD0202904JSKLCCM-2022-02-00882025033Xi WShen LGu XLuo ZBai YZhou ZRen HLiu XHan BBian LHuang WXiang XWang YYao HfalseMembrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice.Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision-tuning of the function of astrocytic processes is garnering increasing attention because of its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca²⁺) transients in astrocytic processes are pivotal for the functional regulation of these processes. However, the understanding of the alterations and regulatory mechanism of microdomain Ca²⁺ transients during stroke remains limited. In the present study, a fast high-resolution, miniaturized two-photon microscopy is used to show that the levels of astrocytic microdomain Ca²⁺ transients are significantly reduced in the peri-infarct area of awake ischemic stroke mice. This2024-01-01T00:00:00Z2024 Dec2026-06-03T00:54:53.851Z2025-04-06T15:08:21.611ZS-EPMC116334883944407810.1002/advs.202404391