biostudies-literatureChen HHNational Science and Technology Council2559-2573https://www.ebi.ac.uk/biostudies/studies/S-EPMC11634477biostudies-literatureUnknown15(6)<h4>Background</h4>Nuclear receptor interaction protein (NRIP) is versatile and engages with various proteins to execute its diverse biological function. NRIP deficiency was reported to cause small myofibre size in adult muscle regeneration, indicating a crucial role of NRIP in myoblast fusion.<h4>Methods</h4>The colocalization and interaction of NRIP with actin were investigated by immunofluorescence and immunoprecipitation assay, respectively. The participation of NRIP in myoblast fusion was demonstrated by cell fusion assay and time-lapse microscopy. The NRIP mutants were generated for mechanism study in NRIP-null C2C12 (termed KO19) cells and muscle-specific NRIP knockout (NRIP cKO) mice. A GEO profile database was used to analyse NRIP expression in Duchenne muscular dystrophy (DMD) paJournal of cachexia, sarcopenia and muscleThe Innovative Role of Nuclear Receptor Interaction Protein in Orchestrating Invadosome Formation for Myoblast Fusion.PMC11634477MOST 111-2320-B-002-077-MY3MOST 110-2320-B-002-052-MY3MOST 104-2320-B-002-053-MY3MOST 107‐2320‐B‐002‐012‐MY3MOST 107-2320-B-002-012-MY3MOST 110‐2320‐B‐002‐052‐MY3MOST 111‐2320‐B‐002‐077‐MY3National Health Research InstituteMOST 104‐2320‐B‐002‐053‐MY3NHRI-105-1053ISINHRI‐105‐1053ISIChen SLHuang YHHsu WETsai LKTsao YPChen HHLai HJHan YJLiu YHHuang HPLin CYfalseThe Innovative Role of Nuclear Receptor Interaction Protein in Orchestrating Invadosome Formation for Myoblast Fusion.<h4>Background</h4>Nuclear receptor interaction protein (NRIP) is versatile and engages with various proteins to execute its diverse biological function. NRIP deficiency was reported to cause small myofibre size in adult muscle regeneration, indicating a crucial role of NRIP in myoblast fusion.<h4>Methods</h4>The colocalization and interaction of NRIP with actin were investigated by immunofluorescence and immunoprecipitation assay, respectively. The participation of NRIP in myoblast fusion was demonstrated by cell fusion assay and time-lapse microscopy. The NRIP mutants were generated for mechanism study in NRIP-null C2C12 (termed KO19) cells and muscle-specific NRIP knockout (NRIP cKO) mice. A GEO profile database was used to analyse NRIP expression in Duchenne muscular dystrophy (DMD) pa2024-01-01T00:00:00Z2024 Dec2026-06-03T00:55:15.4Z2025-04-06T15:09:17.119ZS-EPMC116344773932308810.1002/jcsm.13598