{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim J"],"funding":["National Research Foundation of Korea","Seoul National University Bundang Hospital research fund"],"pagination":["1991"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11639892"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(23)"],"pubmed_abstract":["To develop in vitro respiratory models, it is crucial to identify the factors involved in epithelial cell differentiation. In this study, we comprehensively analyzed the effects of air-liquid interface (ALI) culture on epithelial cell differentiation using single-cell RNA sequencing (scRNA-seq). ALI culture induced a pronounced shift in cell composition, marked by a fivefold increase in ciliated cells and a reduction of more than half in basal cells. Transcriptional signatures associated with epithelial cell differentiation, analyzed using iPathwayGuide software, revealed the downregulation of <i>VEGFA</i> and upregulation of <i>CDKN1A</i> as key signals for epithelial differentiation. Our findings highlight the efficacy of the ALI culture for replicating the human lung airway epithelium a"],"journal":["Cells"],"pubmed_title":["Transcriptomic Analysis of Air-Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation."],"pmcid":["PMC11639892"],"funding_grant_id":["02-2023-0049","No. 02-2023-0049","2021K1A4A7A02097757"],"pubmed_authors":["Kim B","Koo BK","Eo EY","Kim HJ","Cho YJ","Lee H","Kim J","Cho S"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic Analysis of Air-Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation.","description":"To develop in vitro respiratory models, it is crucial to identify the factors involved in epithelial cell differentiation. In this study, we comprehensively analyzed the effects of air-liquid interface (ALI) culture on epithelial cell differentiation using single-cell RNA sequencing (scRNA-seq). ALI culture induced a pronounced shift in cell composition, marked by a fivefold increase in ciliated cells and a reduction of more than half in basal cells. Transcriptional signatures associated with epithelial cell differentiation, analyzed using iPathwayGuide software, revealed the downregulation of <i>VEGFA</i> and upregulation of <i>CDKN1A</i> as key signals for epithelial differentiation. Our findings highlight the efficacy of the ALI culture for replicating the human lung airway epithelium a","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-07-09T10:29:40.911Z","creation":"2025-04-04T01:26:11.206Z"},"accession":"S-EPMC11639892","cross_references":{"pubmed":["39682739"],"doi":["10.3390/cells13231991"]}}