{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Adenuga BM"],"funding":["Fundação para a Ciência e Tecnologia","National Science Centre"],"pagination":["3728"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11640146"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(23)"],"pubmed_abstract":["Roe deer meat is a prized game product in many European countries. However, concerns exist regarding the accuracy of the amount of declared roe deer in processed game meat foods. This study aimed to develop a reliable method for the detection and quantification of roe deer in commercialized game meat products. A TaqMan probe-based quantitative real-time PCR (qPCR) assay was designed, targeting a single-copy 120-bp region of the roe deer agouti signaling protein (ASIP) encoding gene. The method employed the normalized ∆Cq approach to establish a calibration curve for roe deer detection and quantification within 0.05-50% (<i>w</i>/<i>w</i>) in complex raw and processed matrices. The method proved to be specific for roe deer identification, achieving limits of detection and quantification of "],"journal":["Foods (Basel, Switzerland)"],"pubmed_title":["A Novel Normalized Quantitative Real-Time PCR Approach for Ensuring Roe Deer (<i>Capreolus capreolus</i>) Meat Authenticity in Game Meat Foods."],"pmcid":["PMC11640146"],"funding_grant_id":["2021.03670.CEECIND/CP1662/CT0011","2023.07644.CEECIND","2021.03583.CEECIND/CP1662/CT0012","2020/37/B/NZ9/00082","UIDB/50006/2020"],"pubmed_authors":["Mafra I","Biltes R","Spychaj A","Villa C","Costa J","Montowska M","Adenuga BM"],"additional_accession":[]},"is_claimable":false,"name":"A Novel Normalized Quantitative Real-Time PCR Approach for Ensuring Roe Deer (<i>Capreolus capreolus</i>) Meat Authenticity in Game Meat Foods.","description":"Roe deer meat is a prized game product in many European countries. However, concerns exist regarding the accuracy of the amount of declared roe deer in processed game meat foods. This study aimed to develop a reliable method for the detection and quantification of roe deer in commercialized game meat products. A TaqMan probe-based quantitative real-time PCR (qPCR) assay was designed, targeting a single-copy 120-bp region of the roe deer agouti signaling protein (ASIP) encoding gene. The method employed the normalized ∆Cq approach to establish a calibration curve for roe deer detection and quantification within 0.05-50% (<i>w</i>/<i>w</i>) in complex raw and processed matrices. The method proved to be specific for roe deer identification, achieving limits of detection and quantification of ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-22T03:16:27.989Z","creation":"2025-04-04T00:36:54.616Z"},"accession":"S-EPMC11640146","cross_references":{"pubmed":["39682800"],"doi":["10.3390/foods13233728"]}}