biostudies-literatureHe JInnovative Training Program of Zhejiang A & F University12820https://www.ebi.ac.uk/biostudies/studies/S-EPMC11640966biostudies-literatureUnknown25(23)Soil salinization is one of the abiotic stresses that inhibit plant growth and development, which seriously restricts global crop production. WRKY transcription factors play an important role in regulating plant responses to stress such as salt stress. In our previous study, two WRKY family genes from okra, <i>AeWRKY32</i> and <i>AeWRKY70</i>, were significantly up-regulated and down-regulated, respectively, in response to salt stress. In this study, subcellular localization showed that they were localized to the nucleus. The down-regulation of <i>AeWRKY32</i> and <i>AeWRKY70</i> via whole plant virus-induced gene silencing (VIGS) increased and decreased plant sensitivity to salt stress, respectively. Ectopic expression of <i>AeWRKY32</i> and <i>AeWRKY70</i> led to promoted and reduced salInternational journal of molecular sciencesOkra WRKY Transcription Factor AeWRKY32 and AeWRKY70 Are Involved in Salt Stress Response.PMC116409662024kx0001Li XWu JZheng YHe JLi FChen SChen RZhan YfalseOkra WRKY Transcription Factor AeWRKY32 and AeWRKY70 Are Involved in Salt Stress Response.Soil salinization is one of the abiotic stresses that inhibit plant growth and development, which seriously restricts global crop production. WRKY transcription factors play an important role in regulating plant responses to stress such as salt stress. In our previous study, two WRKY family genes from okra, <i>AeWRKY32</i> and <i>AeWRKY70</i>, were significantly up-regulated and down-regulated, respectively, in response to salt stress. In this study, subcellular localization showed that they were localized to the nucleus. The down-regulation of <i>AeWRKY32</i> and <i>AeWRKY70</i> via whole plant virus-induced gene silencing (VIGS) increased and decreased plant sensitivity to salt stress, respectively. Ectopic expression of <i>AeWRKY32</i> and <i>AeWRKY70</i> led to promoted and reduced sal2024-01-01T00:00:00Z2024 Nov2025-04-03T23:26:24.575Z2025-04-03T23:26:24.575ZS-EPMC116409663968453010.3390/ijms252312820