biostudies-literatureOeller MBundesland Salzburg12589https://www.ebi.ac.uk/biostudies/studies/S-EPMC11641817biostudies-literatureUnknown25(23)In regenerative medicine, stromal cells are supposed to play an important role by modulating immune responses and differentiating into various tissue types. The aim of this study was to investigate the influence of heparin, frequently used as an anticoagulant in human platelet lysate (HPL)-supplemented cell cultures, on the expression of non-coding RNA species, particularly microRNAs (miRNA), which are pivotal regulators of gene expression. Through genomic analysis and quantitative RT-PCR, we assessed the differential impact of heparin on miRNA expression in various stromal cell types, derived from human bone marrow, umbilical cord and white adipose tissue. Our results demonstrate that heparin significantly alters miRNA expression, with distinct up- and downregulation patterns depending onInternational journal of molecular sciencesHeparin Differentially Regulates the Expression of Specific miRNAs in Mesenchymal Stromal Cells.PMC1164181720204-WISS/225/197-2019, 20102-F1901166-KZP and 20204-WISS/263/6-6022Rohde ESchally TZimmermann GLauth WSchallmoser KOeller MLaner-Plamberger SfalseHeparin Differentially Regulates the Expression of Specific miRNAs in Mesenchymal Stromal Cells.In regenerative medicine, stromal cells are supposed to play an important role by modulating immune responses and differentiating into various tissue types. The aim of this study was to investigate the influence of heparin, frequently used as an anticoagulant in human platelet lysate (HPL)-supplemented cell cultures, on the expression of non-coding RNA species, particularly microRNAs (miRNA), which are pivotal regulators of gene expression. Through genomic analysis and quantitative RT-PCR, we assessed the differential impact of heparin on miRNA expression in various stromal cell types, derived from human bone marrow, umbilical cord and white adipose tissue. Our results demonstrate that heparin significantly alters miRNA expression, with distinct up- and downregulation patterns depending on2024-01-01T00:00:00Z2024 Nov2025-04-18T13:02:44.273Z2025-04-04T02:14:24.276ZS-EPMC116418173968430110.3390/ijms252312589