{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Nicoletti C"],"funding":["NIAMS NIH HHS","NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["MYOD is an E-box sequence-specific basic Helix-Loop-Helix (bHLH) transcriptional activator that, when expressed in non-muscle cells, induces nuclear reprogramming toward skeletal myogenesis by promoting chromatin accessibility at previously silent loci. Here, we report on the identification of a previously unrecognized property of MYOD as repressor of gene expression, via E-box-independent chromatin binding within accessible genomic elements, which invariably leads to reduced chromatin accessibility. MYOD-mediated repression requires the integrity of functional domains previously implicated in MYOD-mediated activation of gene expression. Repression of mitogen- and growth factor-responsive genes occurs through promoter binding and requires a highly conserved domain within the first helix. R"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.12.05.627024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11643108"],"repository":["biostudies-literature"],"pubmed_title":["E-box independent chromatin recruitment turns MYOD into a transcriptional repressor."],"pmcid":["PMC11643108"],"funding_grant_id":["R01 GM134712","S10 OD026929","R01 AR056712","R01 GM137117","R01 AR045203"],"pubmed_authors":["Nicolau M","Sundar S","Xu M","Massenet J","Pintado-Urbanc AP","Zhang W","Puri PL","Connor LJ","Fang Z","Nicoletti C","Chan TCI","Tapscott SJ","Cheung TH","Caputo L","Schmitt A","Simon MD"],"additional_accession":[]},"is_claimable":false,"name":"E-box independent chromatin recruitment turns MYOD into a transcriptional repressor.","description":"MYOD is an E-box sequence-specific basic Helix-Loop-Helix (bHLH) transcriptional activator that, when expressed in non-muscle cells, induces nuclear reprogramming toward skeletal myogenesis by promoting chromatin accessibility at previously silent loci. Here, we report on the identification of a previously unrecognized property of MYOD as repressor of gene expression, via E-box-independent chromatin binding within accessible genomic elements, which invariably leads to reduced chromatin accessibility. MYOD-mediated repression requires the integrity of functional domains previously implicated in MYOD-mediated activation of gene expression. Repression of mitogen- and growth factor-responsive genes occurs through promoter binding and requires a highly conserved domain within the first helix. R","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-04-08T18:47:09.771Z","creation":"2025-04-06T22:28:24.471Z"},"accession":"S-EPMC11643108","cross_references":{"pubmed":["39677796"],"doi":["10.1101/2024.12.05.627024"]}}