{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Liu HN"],"funding":["NIAID NIH HHS","NLM NIH HHS"],"pubmed_abstract":["<h4>Background & aims</h4>Integrated HBV DNA (iDNA) plays a critical role in HBV pathogenesis, particularly in predicting treatment response and HCC. This study aimed to use an HBV hybridization-capture next-generation sequencing (HBV-NGS) assay to detect HBV-host junction sequences (HBV-JS) in a sensitive nonbiased manner to detect and estimate the iDNA fraction in tissue biopsies and HBV genetics by liquid biopsy.<h4>Methods</h4>HBV DNA from plasmid monomers, HBV-HCC cell line (SNU398, Hep3B, and PLC/PRF/5), tissue biopsies of patients with serum HBV DNA <4 log IU/ml, and matched urine and plasma of HBV patients were assessed by HBV-NGS. Junction-specific qPCR (JS-qPCR) assays were developed to quantify abundant HBV-JS.<h4>Results</h4>We demonstrated high coverage uniformity, reproducibi"],"journal":["medRxiv : the preprint server for health sciences"],"pagination":["2024.12.04.24318256"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11643158"],"repository":["biostudies-literature"],"pubmed_title":["Integrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assay."],"pmcid":["PMC11643158"],"funding_grant_id":["T15 LM007059","R43 AI167169","R56 AI179574","R43 AI174349"],"pubmed_authors":["Liu HN","Shieh FS","Ramirez R","Kim ES","Su YH","Heimer Z","Kubas R","Liu Y","Chang TT","Lau DTY","Guo H","Wang Z","Lin SY","Chen SE"],"additional_accession":[]},"is_claimable":false,"name":"Integrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assay.","description":"<h4>Background & aims</h4>Integrated HBV DNA (iDNA) plays a critical role in HBV pathogenesis, particularly in predicting treatment response and HCC. This study aimed to use an HBV hybridization-capture next-generation sequencing (HBV-NGS) assay to detect HBV-host junction sequences (HBV-JS) in a sensitive nonbiased manner to detect and estimate the iDNA fraction in tissue biopsies and HBV genetics by liquid biopsy.<h4>Methods</h4>HBV DNA from plasmid monomers, HBV-HCC cell line (SNU398, Hep3B, and PLC/PRF/5), tissue biopsies of patients with serum HBV DNA <4 log IU/ml, and matched urine and plasma of HBV patients were assessed by HBV-NGS. Junction-specific qPCR (JS-qPCR) assays were developed to quantify abundant HBV-JS.<h4>Results</h4>We demonstrated high coverage uniformity, reproducibi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-05-27T03:16:18.833Z","creation":"2025-04-04T21:45:41.071Z"},"accession":"S-EPMC11643158","cross_references":{"pubmed":["39677449"],"doi":["10.1101/2024.12.04.24318256"]}}