biostudies-literatureUnknownLiu HNNIAID NIH HHSNLM NIH HHS<h4>Background & aims</h4>Integrated HBV DNA (iDNA) plays a critical role in HBV pathogenesis, particularly in predicting treatment response and HCC. This study aimed to use an HBV hybridization-capture next-generation sequencing (HBV-NGS) assay to detect HBV-host junction sequences (HBV-JS) in a sensitive nonbiased manner to detect and estimate the iDNA fraction in tissue biopsies and HBV genetics by liquid biopsy.<h4>Methods</h4>HBV DNA from plasmid monomers, HBV-HCC cell line (SNU398, Hep3B, and PLC/PRF/5), tissue biopsies of patients with serum HBV DNA <4 log IU/ml, and matched urine and plasma of HBV patients were assessed by HBV-NGS. Junction-specific qPCR (JS-qPCR) assays were developed to quantify abundant HBV-JS.<h4>Results</h4>We demonstrated high coverage uniformity, reproducibimedRxiv : the preprint server for health sciences2024.12.04.24318256https://www.ebi.ac.uk/biostudies/studies/S-EPMC11643158biostudies-literatureIntegrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assay.PMC11643158T15 LM007059R43 AI167169R56 AI179574R43 AI174349Liu HNShieh FSRamirez RKim ESSu YHHeimer ZKubas RLiu YChang TTLau DTYGuo HWang ZLin SYChen SEfalseIntegrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assay.<h4>Background & aims</h4>Integrated HBV DNA (iDNA) plays a critical role in HBV pathogenesis, particularly in predicting treatment response and HCC. This study aimed to use an HBV hybridization-capture next-generation sequencing (HBV-NGS) assay to detect HBV-host junction sequences (HBV-JS) in a sensitive nonbiased manner to detect and estimate the iDNA fraction in tissue biopsies and HBV genetics by liquid biopsy.<h4>Methods</h4>HBV DNA from plasmid monomers, HBV-HCC cell line (SNU398, Hep3B, and PLC/PRF/5), tissue biopsies of patients with serum HBV DNA <4 log IU/ml, and matched urine and plasma of HBV patients were assessed by HBV-NGS. Junction-specific qPCR (JS-qPCR) assays were developed to quantify abundant HBV-JS.<h4>Results</h4>We demonstrated high coverage uniformity, reproducibi2024-01-01T00:00:00Z2024 Dec2026-05-27T03:16:18.833Z2025-04-04T21:45:41.071ZS-EPMC116431583967744910.1101/2024.12.04.24318256