{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ramseier NT"],"pubmed_abstract":["Tetraspanin proteins are closely associated with high-curvature membrane structures and play key roles in organizing membrane domains and regulating membrane signaling in immune cells. However, their specific roles in regulating T cell membrane signaling, particularly within the microvilli often characteristic of these cells, remain poorly understood. Here, we used Jurkat T cells as a model system and investigated CD81 as a member of the tetraspanin family. Using total internal reflection fluorescence (TIRF) microscopy and structured illumination microscopy (SIM), we identified an enrichment of the tetraspanin CD81 microdomains along the actin-rich membrane microvilli. At the distal end of the microvilli, SIM images revealed the spatial colocalization of CD81 with T cell receptors (TCR) an"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.12.07.627345"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11643289"],"repository":["biostudies-literature"],"pubmed_title":["Superresolution Imaging Reveals the Spatial Organization of CD81 Microdomains in Regulating Membrane Signaling on Jurkat T Cell Microvilli."],"pmcid":["PMC11643289"],"pubmed_authors":["Jing H","Anderson J","Hu YS","Ramseier NT"],"additional_accession":[]},"is_claimable":false,"name":"Superresolution Imaging Reveals the Spatial Organization of CD81 Microdomains in Regulating Membrane Signaling on Jurkat T Cell Microvilli.","description":"Tetraspanin proteins are closely associated with high-curvature membrane structures and play key roles in organizing membrane domains and regulating membrane signaling in immune cells. However, their specific roles in regulating T cell membrane signaling, particularly within the microvilli often characteristic of these cells, remain poorly understood. Here, we used Jurkat T cells as a model system and investigated CD81 as a member of the tetraspanin family. Using total internal reflection fluorescence (TIRF) microscopy and structured illumination microscopy (SIM), we identified an enrichment of the tetraspanin CD81 microdomains along the actin-rich membrane microvilli. At the distal end of the microvilli, SIM images revealed the spatial colocalization of CD81 with T cell receptors (TCR) an","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-05T09:51:41.553Z","creation":"2025-04-05T09:51:41.553Z"},"accession":"S-EPMC11643289","cross_references":{"pubmed":["39677771"],"doi":["10.1101/2024.12.07.627345"]}}