{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kang B"],"funding":["Agricultural Science and Technology Innovation Program","China Agriculture Research System of MOF and MARA","National Natural Science Foundation of China"],"pagination":["3431"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11644367"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(23)"],"pubmed_abstract":["Potyviral P3 is involved in viral replication, movement, and pathogenicity; however, its biochemical function is unknown. In this study, the P3 of the zucchini yellow mosaic virus (ZYMV) interacted with ClBBD, a protein with high ortholog bifunctional nuclease activity, in watermelon. The binding site was shown via yeast two-hybrid screening and BiFC assay to be located at the N-terminus of P3 rather than P3N-PIPO. ClBBD localized predominantly to the chloroplast and plasma membrane. ZYMV P3 was also present in the nucleus and cytoplasm as aggregates. When co-expressed with P3 in tobacco, ClBBD formed aggregates with P3 in the cytoplasm. The knockdown of <i>ClBBD</i> using the VIGS vector pV190 and challenge with ZYMV revealed a positive correlation between viral accumulation and ClBBD exp"],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3."],"pmcid":["PMC11644367"],"funding_grant_id":["CARS-25","CAAS-ASTIP-2022-ZFRI-09","U21A20229","31701942"],"pubmed_authors":["Kang B","Liu L","Liu M","Peng B","Liang Z","Zang Y","Gu Q","Liu F","Wu H"],"additional_accession":[]},"is_claimable":false,"name":"A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3.","description":"Potyviral P3 is involved in viral replication, movement, and pathogenicity; however, its biochemical function is unknown. In this study, the P3 of the zucchini yellow mosaic virus (ZYMV) interacted with ClBBD, a protein with high ortholog bifunctional nuclease activity, in watermelon. The binding site was shown via yeast two-hybrid screening and BiFC assay to be located at the N-terminus of P3 rather than P3N-PIPO. ClBBD localized predominantly to the chloroplast and plasma membrane. ZYMV P3 was also present in the nucleus and cytoplasm as aggregates. When co-expressed with P3 in tobacco, ClBBD formed aggregates with P3 in the cytoplasm. The knockdown of <i>ClBBD</i> using the VIGS vector pV190 and challenge with ZYMV revealed a positive correlation between viral accumulation and ClBBD exp","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-04-23T03:24:52.413Z","creation":"2025-04-04T01:54:38.134Z"},"accession":"S-EPMC11644367","cross_references":{"pubmed":["39683224"],"doi":["10.3390/plants13233431"]}}