biostudies-literatureChoi YJUniversity of MichiganNIDDK NIH HHSNHLBI NIH HHSJDRFNIDDKBoettcher Foundation and Intramural Research Program at NIDDK and CDCe183984https://www.ebi.ac.uk/biostudies/studies/S-EPMC11645151biostudies-literatureUnknown134(24)BACKGROUNDIn type 1 diabetes (T1D), impaired insulin sensitivity may contribute to the development of diabetic kidney disease (DKD) through alterations in kidney oxidative metabolism.METHODSYoung adults with T1D (n = 30) and healthy controls (HCs) (n = 20) underwent hyperinsulinemic-euglycemic clamp studies, MRI, 11C-acetate PET, kidney biopsies, single-cell RNA-Seq, and spatial metabolomics to assess this relationship.RESULTSParticipants with T1D had significantly higher glomerular basement membrane (GBM) thickness compared with HCs. T1D participants exhibited lower insulin sensitivity and cortical oxidative metabolism, correlating with higher insulin sensitivity. Proximal tubular transcripts of TCA cycle and oxidative phosphorylation enzymes were lower in T1D. Spatial metabolomics showedThe Journal of clinical investigationAttenuated kidney oxidative metabolism in young adults with type 1 diabetes.PMC116451512-SRA-2019-845-S-BP30 DK116073L40 HL159798R01 DK129211P30 DK020572R01 HL16543321FED2100157DPGP30 DK081943K23 HL159292R01 DK137844R01 DK132399K23 DK125529K08 DK124449Zhang GBrosius FCTamayo IMPuelles VGGoodrich JAAlakwaa FChin BBKendrick JDemeke DSPrasad PGurung BKNair VMacDonald ASaulnier PJSchaub JAPyle LStreet KCherney DZde Boer IHNaik ASFlynn MVenkatachalam MAShah VNGross Svan Raalte DHNelson RGSnell-Bergeon JKHodgin JBOtto EARichard GSharma KBlondin DPTommerdahl KDriscoll LYang YLadd PMcCown PJKretzler MNadeau KJChoi YJLi LPRuiz ANarongkiatikhun PBjornstad PBirznieks CVigers TfalseAttenuated kidney oxidative metabolism in young adults with type 1 diabetes.BACKGROUNDIn type 1 diabetes (T1D), impaired insulin sensitivity may contribute to the development of diabetic kidney disease (DKD) through alterations in kidney oxidative metabolism.METHODSYoung adults with T1D (n = 30) and healthy controls (HCs) (n = 20) underwent hyperinsulinemic-euglycemic clamp studies, MRI, 11C-acetate PET, kidney biopsies, single-cell RNA-Seq, and spatial metabolomics to assess this relationship.RESULTSParticipants with T1D had significantly higher glomerular basement membrane (GBM) thickness compared with HCs. T1D participants exhibited lower insulin sensitivity and cortical oxidative metabolism, correlating with higher insulin sensitivity. Proximal tubular transcripts of TCA cycle and oxidative phosphorylation enzymes were lower in T1D. Spatial metabolomics showed2024-01-01T00:00:00Z2024 Oct2026-06-03T03:08:03.816Z2025-04-04T01:54:29.228ZS-EPMC116451513943669510.1172/JCI183984