{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mevissen TET"],"funding":["American Cancer Society","EMBO","Howard Hughes Medical Institute","NIEHS NIH HHS","NHLBI NIH HHS","National Institutes of Health"],"pagination":["7091-7106.e24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11645862"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["187(25)"],"pubmed_abstract":["In transcription-coupled nucleotide excision repair (TC-NER), stalled RNA polymerase II (RNA Pol II) binds CSB and CRL4<sup>CSA</sup>, which cooperate with UVSSA and ELOF1 to recruit TFIIH. To explore the mechanism of TC-NER, we recapitulated this reaction in vitro. When a plasmid containing a site-specific lesion is transcribed in frog egg extract, error-free repair is observed that depends on CSB, CRL4<sup>CSA</sup>, UVSSA, and ELOF1. Repair also requires STK19, a factor previously implicated in transcription recovery after UV exposure. A 1.9-Å cryo-electron microscopy structure shows that STK19 binds the TC-NER complex through CSA and the RPB1 subunit of RNA Pol II. Furthermore, AlphaFold predicts that STK19 interacts with the XPD subunit of TFIIH, and disrupting this interface impairs "],"journal":["Cell"],"pubmed_title":["STK19 positions TFIIH for cell-free transcription-coupled DNA repair."],"pmcid":["PMC11645862"],"funding_grant_id":["R01 HL098316","DP2 ES036404"],"pubmed_authors":["Schmid EW","Walter JC","Mevissen TET","Farnung L","Kummecke M"],"additional_accession":[]},"is_claimable":false,"name":"STK19 positions TFIIH for cell-free transcription-coupled DNA repair.","description":"In transcription-coupled nucleotide excision repair (TC-NER), stalled RNA polymerase II (RNA Pol II) binds CSB and CRL4<sup>CSA</sup>, which cooperate with UVSSA and ELOF1 to recruit TFIIH. To explore the mechanism of TC-NER, we recapitulated this reaction in vitro. When a plasmid containing a site-specific lesion is transcribed in frog egg extract, error-free repair is observed that depends on CSB, CRL4<sup>CSA</sup>, UVSSA, and ELOF1. Repair also requires STK19, a factor previously implicated in transcription recovery after UV exposure. A 1.9-Å cryo-electron microscopy structure shows that STK19 binds the TC-NER complex through CSA and the RPB1 subunit of RNA Pol II. Furthermore, AlphaFold predicts that STK19 interacts with the XPD subunit of TFIIH, and disrupting this interface impairs ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-06-02T10:45:48.794Z","creation":"2025-04-04T01:54:56.636Z"},"accession":"S-EPMC11645862","cross_references":{"pubmed":["39547228"],"doi":["10.1016/j.cell.2024.10.020"]}}