{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["Haile S"],"pubmed_abstract":["The advent of long-read (LR) sequencing technologies has provided a direct opportunity to determine the structure of transcripts with potential for end-to-end sequencing of full-length RNAs. LR methods that have been described to date include commercial offerings from Oxford Nanopore Technologies (ONT) and Pacific Biosciences. These kits are based on selection of polyadenylated (polyA+) RNAs and/or oligo-dT priming of reverse transcription. Thus, these approaches do not allow comprehensive interrogation of the transcriptome due to their exclusion of non-polyadenylated (polyA-) RNAs. In addition, polyA + specificity also results in 3'-biased measurements of PolyA+ RNAs especially when the RNA input is partially degraded. To address these limitations of current LR protocols, we modified rRNA"],"journal":["Frontiers in genetics"],"pagination":["1466338"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11647301"],"repository":["biostudies-literature"],"pubmed_title":["Adaptable and comprehensive approaches for long-read nanopore sequencing of polyadenylated and non-polyadenylated RNAs."],"pmcid":["PMC11647301"],"pubmed_authors":["O'Neill K","Moore RA","Mungall AJ","Bayega A","Bala M","Pandoh PK","Ma Y","Chuah E","Mungall KL","Xu J","Zhao Y","Coope RJN","Marra MA","Corbett RD","Jones SJM","Haile S","Smailus DE"],"additional_accession":[]},"is_claimable":false,"name":"Adaptable and comprehensive approaches for long-read nanopore sequencing of polyadenylated and non-polyadenylated RNAs.","description":"The advent of long-read (LR) sequencing technologies has provided a direct opportunity to determine the structure of transcripts with potential for end-to-end sequencing of full-length RNAs. LR methods that have been described to date include commercial offerings from Oxford Nanopore Technologies (ONT) and Pacific Biosciences. These kits are based on selection of polyadenylated (polyA+) RNAs and/or oligo-dT priming of reverse transcription. Thus, these approaches do not allow comprehensive interrogation of the transcriptome due to their exclusion of non-polyadenylated (polyA-) RNAs. In addition, polyA + specificity also results in 3'-biased measurements of PolyA+ RNAs especially when the RNA input is partially degraded. To address these limitations of current LR protocols, we modified rRNA","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-18T14:40:10.192Z","creation":"2025-04-07T00:55:46.874Z"},"accession":"S-EPMC11647301","cross_references":{"pubmed":["39687742"],"doi":["10.3389/fgene.2024.1466338"]}}