{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Simon L"],"funding":["Bundesministerium für Bildung und Forschung","Deutscher Akademischer Austauschdienst","Thüringer Aufbaubank","Deutsche Forschungsgemeinschaft","Agence Nationale de la Recherche","European Regional Development Fund"],"pagination":["e2400148"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11648592"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(12)"],"pubmed_abstract":["Due to the increasing challenges posed by the growing immunity to poly(ethylene glycol) (PEG), there is growing interest in innovative polymer-based materials as viable alternatives. In this study, the advantages of lipids and polymers are combined to allow efficient and rapid cytoplasmic drug delivery. Specifically, poly(2-methyl-2-oxazoline) is modified with a cholesteryl hemisuccinate group as a lipid anchor (CHEMSPOx). The CHEMSPOx is additionally functionalized with a coumarin group (CHEMSPOx-coumarin). Both polymers self-assembled in water into vesicles of ≈100 nm and are successfully loaded with a hydrophobic model drug. The loaded vesicles reveal high cellular internalization across variant cell lines within 1 h at 37 °C as well as 4 °C, albeit to a lesser extent. A kinetic study confirms the fast internalization within 5 min after the sample's addition. Therefore, different internalization pathways are involved, e.g., active uptake but also nonenergy dependent mechanisms. CHEMSPOx and CHEMSPOx-coumarin further demonstrate excellent cyto-, hemo-, and membrane compatibility, as well as a membrane-protecting effect, which underlines their good safety profile for potential biological intravenous application. Overall, CHEMSPOx, as a lipopolyoxazoline, holds great potential for versatile biological applications such as fast and direct intracellular delivery or cellular lysis protection."],"journal":["Macromolecular bioscience"],"pubmed_title":["Polyoxazolines with Cholesterol Lipid Anchor for Fast Intracellular Delivery."],"pmcid":["PMC11648592"],"funding_grant_id":["ANR-20-CE09-0011-01","358263073","2018FGI0025","13XP5034A","316213987","2021 FGI 0005","57604510","514006196"],"pubmed_authors":["Eberhardt J","Simon L","Brendel JC","Reichel LS","Morille M","Schubert US","Devoisselle JM","Traeger A","Catrouillet S","Hoeppener S","Lapinte V","Benkhaled BT"],"additional_accession":[]},"is_claimable":false,"name":"Polyoxazolines with Cholesterol Lipid Anchor for Fast Intracellular Delivery.","description":"Due to the increasing challenges posed by the growing immunity to poly(ethylene glycol) (PEG), there is growing interest in innovative polymer-based materials as viable alternatives. In this study, the advantages of lipids and polymers are combined to allow efficient and rapid cytoplasmic drug delivery. Specifically, poly(2-methyl-2-oxazoline) is modified with a cholesteryl hemisuccinate group as a lipid anchor (CHEMSPOx). The CHEMSPOx is additionally functionalized with a coumarin group (CHEMSPOx-coumarin). Both polymers self-assembled in water into vesicles of ≈100 nm and are successfully loaded with a hydrophobic model drug. The loaded vesicles reveal high cellular internalization across variant cell lines within 1 h at 37 °C as well as 4 °C, albeit to a lesser extent. A kinetic study confirms the fast internalization within 5 min after the sample's addition. Therefore, different internalization pathways are involved, e.g., active uptake but also nonenergy dependent mechanisms. CHEMSPOx and CHEMSPOx-coumarin further demonstrate excellent cyto-, hemo-, and membrane compatibility, as well as a membrane-protecting effect, which underlines their good safety profile for potential biological intravenous application. Overall, CHEMSPOx, as a lipopolyoxazoline, holds great potential for versatile biological applications such as fast and direct intracellular delivery or cellular lysis protection.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-18T12:57:29.375Z","creation":"2025-04-06T22:25:33.517Z"},"accession":"S-EPMC11648592","cross_references":{"pubmed":["39374348"],"doi":["10.1002/mabi.202400148"]}}