{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han Y-L"],"funding":["General Project of Inner Mongolia Medical University","Inner Mongolia \"Prairie Elite\" Project Youth Innovation and Entrepreneurship Talent Training Program","Inner Mongolia “Prairie Elite” Project Youth Innovation and Entrepreneurship Talent Training Program","School Science and Technology Team Project of Inner Mongolia Medcal University"],"pagination":["e0110124"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11651102"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(12)"],"pubmed_abstract":["Carbapenem-resistant hypervirulent <i>Klebsiella pneumoniae</i> (CR-hvKP) strains present a significant global public health threat due to their high mortality rates. This study investigated the genomic characteristics of seven ST11-K1 CR-hvKP isolates harboring highly homologous KPC-2-encoding multidrug-resistance plasmids. The strains were isolated from a Chinese tertiary hospital between 2017 and 2020. Whole-genome sequencing and bioinformatic analysis revealed various antibiotic resistance genes (ARGs) and virulence determinants. The <i>bla</i><sub>KPC-2</sub>-bearing plasmids that contain multiple antibiotic-resistance genes were also identified in these strains. ISfinder and Orifinder were applied to identify insertion sequences (IS) and conjugation-related factors among these <i>bla</i><sub>KPC-2</sub>-bearing plasmids. The <i>bla</i><sub>KPC-2</sub> was highly consistent in seven <i>bla</i><sub>KPC-2</sub>-bearing plasmids (IS<i>Kpn6-bla</i><sub>KPC-2</sub>-IS<i>Kpn27</i>-IS<i>Yps3</i>-IS<i>26</i>). In addition, we found a region composed of IS<i>IR</i>, Tn<i>5393</i>, and IS<i>26</i>. It was located upstream of the <i>bla</i><sub>CTX-M-15</sub> gene and presented in six <i>bla</i><sub>KPC-2</sub>-bearing plasmids, with pCR-hvKP221-KPC-P3 as an exception. Conjugation experiments demonstrated the horizontal transfer of resistance plasmids pCR-hvKP128-KPC-P1 and pCR-hvKP132-KPC-P1 across species. Notably, pLVPK-like virulence plasmids carrying virulence gene clusters pCR-hvKP173-Vir-P1, and pCR-hvKP221-Vir-P1 were also detected. A fusional plasmid pCR-hvKP221-Vir-P2, which carries virulence gene clusters and ARGs, was also identified. Five CR-hvKP strains displayed enhanced biofilm formation and high virulence <i>in vivo</i> infection models. Phylogenetic and single nucleotide polymorphism (SNP) analyses indicated a close genetic relationship among the isolates, suggesting a subclade. These findings highlight the complex genetic profiles and potential transmission mechanisms of CR-hvKP strains.<h4>Importance</h4>We reported seven CR-hvKP strains all carried a highly homologous <i>bla</i><sub>KPC-2</sub> integrated IncFⅡ-resistant plasmid, and two strains harbored virulence plasmids. Conjugation experiments confirmed the transferability of these plasmids, indicating a potential for resistance spread. Phylogenetic analysis clarified the relationship among the CR-hvKP isolates. This study provides insights into the phenotypic and genomic characteristics of seven ST11-K1 CR-hvKP strains. The high prevalence and potential for local outbreaks emphasize the need for effective control measures."],"journal":["mSystems"],"pubmed_title":["Phenotypic and genomic characterization of ST11-K1 CR-hvKP with highly homologous <i>bla</i><sub>KPC-2</sub>-bearing plasmids in China."],"pmcid":["PMC11651102"],"funding_grant_id":["YKD2022TD026","ZY0130013","YKD2021MS011"],"pubmed_authors":["Lv Y-Y","Wen J-X","Wang J-R","Hu Z-D","Zheng W-Q","Zhu H-Z","Zhao W","Han Y-L","Wang H","Wang Y-Y"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypic and genomic characterization of ST11-K1 CR-hvKP with highly homologous <i>bla</i><sub>KPC-2</sub>-bearing plasmids in China.","description":"Carbapenem-resistant hypervirulent <i>Klebsiella pneumoniae</i> (CR-hvKP) strains present a significant global public health threat due to their high mortality rates. This study investigated the genomic characteristics of seven ST11-K1 CR-hvKP isolates harboring highly homologous KPC-2-encoding multidrug-resistance plasmids. The strains were isolated from a Chinese tertiary hospital between 2017 and 2020. Whole-genome sequencing and bioinformatic analysis revealed various antibiotic resistance genes (ARGs) and virulence determinants. The <i>bla</i><sub>KPC-2</sub>-bearing plasmids that contain multiple antibiotic-resistance genes were also identified in these strains. ISfinder and Orifinder were applied to identify insertion sequences (IS) and conjugation-related factors among these <i>bla</i><sub>KPC-2</sub>-bearing plasmids. The <i>bla</i><sub>KPC-2</sub> was highly consistent in seven <i>bla</i><sub>KPC-2</sub>-bearing plasmids (IS<i>Kpn6-bla</i><sub>KPC-2</sub>-IS<i>Kpn27</i>-IS<i>Yps3</i>-IS<i>26</i>). In addition, we found a region composed of IS<i>IR</i>, Tn<i>5393</i>, and IS<i>26</i>. It was located upstream of the <i>bla</i><sub>CTX-M-15</sub> gene and presented in six <i>bla</i><sub>KPC-2</sub>-bearing plasmids, with pCR-hvKP221-KPC-P3 as an exception. Conjugation experiments demonstrated the horizontal transfer of resistance plasmids pCR-hvKP128-KPC-P1 and pCR-hvKP132-KPC-P1 across species. Notably, pLVPK-like virulence plasmids carrying virulence gene clusters pCR-hvKP173-Vir-P1, and pCR-hvKP221-Vir-P1 were also detected. A fusional plasmid pCR-hvKP221-Vir-P2, which carries virulence gene clusters and ARGs, was also identified. Five CR-hvKP strains displayed enhanced biofilm formation and high virulence <i>in vivo</i> infection models. Phylogenetic and single nucleotide polymorphism (SNP) analyses indicated a close genetic relationship among the isolates, suggesting a subclade. These findings highlight the complex genetic profiles and potential transmission mechanisms of CR-hvKP strains.<h4>Importance</h4>We reported seven CR-hvKP strains all carried a highly homologous <i>bla</i><sub>KPC-2</sub> integrated IncFⅡ-resistant plasmid, and two strains harbored virulence plasmids. Conjugation experiments confirmed the transferability of these plasmids, indicating a potential for resistance spread. Phylogenetic analysis clarified the relationship among the CR-hvKP isolates. This study provides insights into the phenotypic and genomic characteristics of seven ST11-K1 CR-hvKP strains. The high prevalence and potential for local outbreaks emphasize the need for effective control measures.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-06-03T05:25:36.628Z","creation":"2025-04-04T21:10:18.547Z"},"accession":"S-EPMC11651102","cross_references":{"pubmed":["39555910"],"doi":["10.1128/msystems.01101-24"]}}