biostudies-literatureMeas SJNIA NIH HHSNIDDK NIH HHSNational Aeronautics and Space AdministrationUBC CIHR SRTCNational Institutes of HealthNASA Goddard Space Flight CenterNIAMS NIH HHSCanadian Institutes of Health Research116973https://www.ebi.ac.uk/biostudies/studies/S-EPMC11651238biostudies-literatureUnknown179Age- and disuse-related bone loss both result in decreases in bone mineral density, cortical thickness, and trabecular thickness and connectivity. Disuse induces changes in the balance of bone formation and bone resorption like those seen with aging. There is a need to experimentally compare these two mechanisms at a structural and transcriptomic level to better understand how they may be similar or different. Bone microarchitecture and biomechanical properties were compared between 6- and 22-month-old C57BL/6 J male control mice and 6-month-old mice that were hindlimb unloaded (HLU) for 3 weeks. Epiphyseal trabecular bone was the compartment most affected by HLU and demonstrated an intermediate bone phenotype between age-matched controls and aged controls. RNA extracted from whole-bone maBoneA comparison of bone microarchitectural and transcriptomic changes in murine long bones in response to hindlimb unloading and aging.PMC11651238R01 AR068132DFSA 45476180NSSC18K1473R01 AG013087R01 DK128552DeNapoli RGhosh PFriedman MAMeas SJDaire GMDonahue HJFarr JNfalseA comparison of bone microarchitectural and transcriptomic changes in murine long bones in response to hindlimb unloading and aging.Age- and disuse-related bone loss both result in decreases in bone mineral density, cortical thickness, and trabecular thickness and connectivity. Disuse induces changes in the balance of bone formation and bone resorption like those seen with aging. There is a need to experimentally compare these two mechanisms at a structural and transcriptomic level to better understand how they may be similar or different. Bone microarchitecture and biomechanical properties were compared between 6- and 22-month-old C57BL/6 J male control mice and 6-month-old mice that were hindlimb unloaded (HLU) for 3 weeks. Epiphyseal trabecular bone was the compartment most affected by HLU and demonstrated an intermediate bone phenotype between age-matched controls and aged controls. RNA extracted from whole-bone ma2024-01-01T00:00:00Z2024 Feb2026-06-01T05:46:03.164Z2025-04-05T10:19:13.303ZS-EPMC116512383799604610.1016/j.bone.2023.116973