biostudies-literatureUnknown28(1)Stanaway IB<h4>Background</h4>Patients with sepsis-induced AKI can be classified into two distinct sub-phenotypes (AKI-SP1, AKI-SP2) that differ in clinical outcomes and response to treatment. The biologic mechanisms underlying these sub-phenotypes remains unknown. Our objective was to understand the underlying biology that differentiates AKI sub-phenotypes and associations with kidney outcomes.<h4>Methods</h4>We prospectively enrolled 173 ICU patients with sepsis from a suspected respiratory infection (87 without AKI and 86 with AKI on enrollment). Among the AKI patients, 66 were classified as AKI-SP1 and 20 as AKI-SP2 using a three-plasma biomarker classifier. Aptamer-based proteomics assessed 5,212 proteins in urine collected on ICU admission. We compared urinary protein abundances between AKI subCritical care (London, England)419https://www.ebi.ac.uk/biostudies/studies/S-EPMC11654061biostudies-literatureUrinary proteomics identifies distinct immunological profiles of sepsis associated AKI sub-phenotypes.PMC11654061Mikacenic CHimmelfarb JStanaway IBWurfel MMSathe NABailey ZLo JZelnick LRMorrell EDBhatraju PKEvans LMabrey FLSpeckmaier SfalseUrinary proteomics identifies distinct immunological profiles of sepsis associated AKI sub-phenotypes.<h4>Background</h4>Patients with sepsis-induced AKI can be classified into two distinct sub-phenotypes (AKI-SP1, AKI-SP2) that differ in clinical outcomes and response to treatment. The biologic mechanisms underlying these sub-phenotypes remains unknown. Our objective was to understand the underlying biology that differentiates AKI sub-phenotypes and associations with kidney outcomes.<h4>Methods</h4>We prospectively enrolled 173 ICU patients with sepsis from a suspected respiratory infection (87 without AKI and 86 with AKI on enrollment). Among the AKI patients, 66 were classified as AKI-SP1 and 20 as AKI-SP2 using a three-plasma biomarker classifier. Aptamer-based proteomics assessed 5,212 proteins in urine collected on ICU admission. We compared urinary protein abundances between AKI sub2024-01-01T00:00:00Z2024 Dec2025-04-19T04:24:51.703Z2025-04-19T04:24:51.703ZS-EPMC116540613969571510.1186/s13054-024-05202-9