biostudies-literatureUnknown5(4)Wu CM2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.STAR protocols103478https://www.ebi.ac.uk/biostudies/studies/S-EPMC11656071biostudies-literatureProtocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.PMC11656071Che TPHsu CNHee SWLiao DChu SLLi HYLiu YWChang YCWu CMYang WSLee HLLee TYLi WYChuang GTLiao KCHsuan CFLee JHfalseProtocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.2024-01-01T00:00:00Z2024 Dec2026-05-29T14:36:40.813Z2025-04-04T00:38:41.042ZS-EPMC116560713964449710.1016/j.xpro.2024.103478