{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gao J"],"funding":["Ocean Negative Carbon Emissions (ONCE) Program","MOST | National Natural Science Foundation of China (NSFC)"],"pagination":["e0077024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11656776"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(12)"],"pubmed_abstract":["16S rRNA gene sequencing is the gold standard for identifying microbial diversity in environmental communities. The Illumina short-read platform is widely used in marine environment studies due to its cost-effectiveness and high accuracy, but its limited read length restricts taxonomic identification mainly to genus or family levels. Recently, the PacBio long-read sequencing platform was developed. This method has exceptional base-level resolution exceeding 99%, thereby effectively mitigating the challenges associated with high error rates commonly observed in long-read sequencing technologies. However, few studies have compared the PacBio long-read and Illumina short-read platforms in marine deep-sea sediments. Here, the PacBio long-read and Illumina short-read platforms were compared wit"],"journal":["mSphere"],"pubmed_title":["Improved resolution of microbial diversity in deep-sea surface sediments using PacBio long-read 16S rRNA gene sequencing."],"pmcid":["PMC11656776"],"funding_grant_id":["42188102, 42293292"],"pubmed_authors":["Cai R","Jiao N","Sa B","Leung EL-H","Gao J","Chen X","Yan Y","Liu D","Wang Z","Deng W","Yan W"],"additional_accession":[]},"is_claimable":false,"name":"Improved resolution of microbial diversity in deep-sea surface sediments using PacBio long-read 16S rRNA gene sequencing.","description":"16S rRNA gene sequencing is the gold standard for identifying microbial diversity in environmental communities. The Illumina short-read platform is widely used in marine environment studies due to its cost-effectiveness and high accuracy, but its limited read length restricts taxonomic identification mainly to genus or family levels. Recently, the PacBio long-read sequencing platform was developed. This method has exceptional base-level resolution exceeding 99%, thereby effectively mitigating the challenges associated with high error rates commonly observed in long-read sequencing technologies. However, few studies have compared the PacBio long-read and Illumina short-read platforms in marine deep-sea sediments. Here, the PacBio long-read and Illumina short-read platforms were compared wit","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-22T10:38:21.49Z","creation":"2025-04-05T23:41:44.767Z"},"accession":"S-EPMC11656776","cross_references":{"pubmed":["39530673"],"doi":["10.1128/msphere.00770-24"]}}