{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lucinskaite E"],"funding":["Bio-Techne and the University of Exeter","Biotechnology and Biological Sciences Research Council"],"pagination":["e2405032"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11657032"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(51)"],"pubmed_abstract":["The DNA-based single molecule super-resolution imaging approach, DNA-PAINT, can achieve nanometer resolution of single targets. However, the approach can suffer from significant non-specific background signals originating from non-specifically bound DNA-conjugated DNA-PAINT secondary antibodies as shown here. Using dye-modified oligonucleotides the location of DNA-PAINT secondary antibody probes can easily be observed with widefield imaging prior to beginning a super-resolution measurement. This reveals that a substantial proportion of DNA probes can accumulate, non-specifically, within the nucleus, as well as across the cytoplasm, of cells. Here, Shielded DNA-PAINT labeling is introduced, a method using partially or fully double-stranded docking strand sequences, prior to labeling, in buf"],"journal":["Small (Weinheim an der Bergstrasse, Germany)"],"pubmed_title":["Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols."],"pmcid":["PMC11657032"],"funding_grant_id":["BB/T007176/1"],"pubmed_authors":["Lucinskaite E","Estell C","Bokhobza AFE","Meletiou A","Clowsley AH","Michele LD","Stannard A","West S","Soeller C"],"additional_accession":[]},"is_claimable":false,"name":"Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols.","description":"The DNA-based single molecule super-resolution imaging approach, DNA-PAINT, can achieve nanometer resolution of single targets. However, the approach can suffer from significant non-specific background signals originating from non-specifically bound DNA-conjugated DNA-PAINT secondary antibodies as shown here. Using dye-modified oligonucleotides the location of DNA-PAINT secondary antibody probes can easily be observed with widefield imaging prior to beginning a super-resolution measurement. This reveals that a substantial proportion of DNA probes can accumulate, non-specifically, within the nucleus, as well as across the cytoplasm, of cells. Here, Shielded DNA-PAINT labeling is introduced, a method using partially or fully double-stranded docking strand sequences, prior to labeling, in buf","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T21:45:38.039Z","creation":"2025-04-04T21:45:38.039Z"},"accession":"S-EPMC11657032","cross_references":{"pubmed":["39422065"],"doi":["10.1002/smll.202405032"]}}