biostudies-literatureZubeldia-Varela EJapan Society for the Promotion of Science (JSPS)Deutscher Akademischer Austausch Dienst (DAAD)FEDER 'Investing in your future' for the thematic network and co-operative research centres RICORS 'Red de Enfermedades Inflamatorias (REI)'Instituto de Salud Carlos IIIEuropean Regional Development Fund3430-3447https://www.ebi.ac.uk/biostudies/studies/S-EPMC11657046biostudies-literatureUnknown79(12)<h4>Background</h4>The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma and anaphylaxis Importantly, high-IgE levels are a poor prognostic factor in gastrointestinal allergies.<h4>Methods</h4>This study investigated how high-IgE levels influence the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. In addition, correlation of the altered metabolome with gut microbiome was analysed.<h4>Results</h4>Ovalbumin-sensitized and egg-white diet-fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulatioAllergyThe impact of high-IgE levels on metabolome and microbiome in experimental allergic enteritis.PMC11657046PI19/00044(RD21/0002/0008)PI20/01366PID2021‐122490NB‐I00PI17/01087PID2021-122490NB-I00Vieths SBarbas CPerez-Gordo MGonzalez-Menendez IMartella MNochi TLaino JYu PBarber DToda MSteigerwald HQuintanilla-Martinez LKrause MZubeldia-Varela EIslam JBlanco-Perez FBarker-Tejeda TCRojo DVillasenor AfalseThe impact of high-IgE levels on metabolome and microbiome in experimental allergic enteritis.<h4>Background</h4>The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma and anaphylaxis Importantly, high-IgE levels are a poor prognostic factor in gastrointestinal allergies.<h4>Methods</h4>This study investigated how high-IgE levels influence the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. In addition, correlation of the altered metabolome with gut microbiome was analysed.<h4>Results</h4>Ovalbumin-sensitized and egg-white diet-fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulatio2024-01-01T00:00:00Z2024 Dec2025-04-18T14:40:12.11Z2025-04-07T00:56:43.927ZS-EPMC116570463893265510.1111/all.16202