biostudies-literatureUnknown23(1)Demisie S<h4>Background</h4>The ongoing emergence and spread of drug-resistant pathogens necessitate urgent solutions. Natural products from bacterial sources are recognized as a promising source of antibiotics. This study aimed to isolate and characterize soil microorganisms from extremely hot environments and to screen their secondary metabolites for antibacterial activity.<h4>Methods</h4>Bacterial isolates were identified using standard culture techniques. Primary and secondary screenings for antimicrobial activity were conducted using the Modified Kirby-Bauer antibiotic susceptibility test against five bacterial species. Based on the efficacy of antimicrobial activity against these target pathogens, the isolate Pseudomonas sp. strain ASTU00105 was selected for further characterization through wMicrobial cell factories332https://www.ebi.ac.uk/biostudies/studies/S-EPMC11657271biostudies-literatureBioprospecting secondary metabolites with antimicrobial properties from soil bacteria in high-temperature ecosystems.PMC11657271Abera ASatessa GDFufa FShenkutie AMTafess KWolday DTasew GDemisie SOh DCfalseBioprospecting secondary metabolites with antimicrobial properties from soil bacteria in high-temperature ecosystems.<h4>Background</h4>The ongoing emergence and spread of drug-resistant pathogens necessitate urgent solutions. Natural products from bacterial sources are recognized as a promising source of antibiotics. This study aimed to isolate and characterize soil microorganisms from extremely hot environments and to screen their secondary metabolites for antibacterial activity.<h4>Methods</h4>Bacterial isolates were identified using standard culture techniques. Primary and secondary screenings for antimicrobial activity were conducted using the Modified Kirby-Bauer antibiotic susceptibility test against five bacterial species. Based on the efficacy of antimicrobial activity against these target pathogens, the isolate Pseudomonas sp. strain ASTU00105 was selected for further characterization through w2024-01-01T00:00:00Z2024 Dec2025-04-04T21:45:00.582Z2025-04-04T21:45:00.582ZS-EPMC116572713969655510.1186/s12934-024-02589-6