biostudies-literatureUnknown1(1)Lerer LThere is growing interest in the therapeutic potential of 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) for psychiatric disorders. Although 5-MeO-DMT can be chemically synthesized, the parotoid gland secretions of Incilius alvarius (also known as the Colorado River or Sonoran Desert toad) contain 5-MeO-DMT and other molecules including bufotenine, bufagenins, bufotoxins, and indole alkylamines that may have individual clinical utility or act as entourage molecules to enhance the activity of 5-MeO-DMT. I. alvarius is currently under severe ecological pressure due to demand for natural 5-MeO-DMT and habitat loss. We established a cell line from tissue obtained by wedge biopsy of the I. alvarius parotoid gland and confirmed the cell-based biosynthesis of 5-MeO-DMT bPsychedelic medicine (New Rochelle, N.Y.)38-42https://www.ebi.ac.uk/biostudies/studies/S-EPMC11658656biostudies-literatureIncilius alvarius Cell-Based Synthesis of 5-Methoxy-N,N-Dimethyltryptamine.PMC11658656Lerer BReynolds EBlakolmer KLerer LVaria JfalseIncilius alvarius Cell-Based Synthesis of 5-Methoxy-N,N-Dimethyltryptamine.There is growing interest in the therapeutic potential of 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) for psychiatric disorders. Although 5-MeO-DMT can be chemically synthesized, the parotoid gland secretions of Incilius alvarius (also known as the Colorado River or Sonoran Desert toad) contain 5-MeO-DMT and other molecules including bufotenine, bufagenins, bufotoxins, and indole alkylamines that may have individual clinical utility or act as entourage molecules to enhance the activity of 5-MeO-DMT. I. alvarius is currently under severe ecological pressure due to demand for natural 5-MeO-DMT and habitat loss. We established a cell line from tissue obtained by wedge biopsy of the I. alvarius parotoid gland and confirmed the cell-based biosynthesis of 5-MeO-DMT b2023-01-01T00:00:00Z2023 Mar2025-04-22T18:48:21.118Z2025-04-06T02:38:20.179ZS-EPMC116586564004701010.1089/psymed.2022.0001