{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramani A"],"funding":["Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research)","Deutsche Forschungsgemeinschaft (German Research Foundation)","Volkswagen Foundation (VolkswagenStiftung)","Fritz Thyssen Stiftung (Fritz Thyssen Foundation)","Deutsche Krebshilfe (German Cancer Aid)"],"pagination":["10703"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11659410"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Brain organoids offer unprecedented insights into brain development and disease modeling and hold promise for drug screening. Significant hindrances, however, are morphological and cellular heterogeneity, inter-organoid size differences, cellular stress, and poor reproducibility. Here, we describe a method that reproducibly generates thousands of organoids across multiple hiPSC lines. These High Quantity brain organoids (Hi-Q brain organoids) exhibit reproducible cytoarchitecture, cell diversity, and functionality, are free from ectopically active cellular stress pathways, and allow cryopreservation and re-culturing. Patient-derived Hi-Q brain organoids recapitulate distinct forms of developmental defects: primary microcephaly due to a mutation in CDK5RAP2 and progeria-associated defects o"],"journal":["Nature communications"],"pubmed_title":["Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening."],"pmcid":["PMC11659410"],"funding_grant_id":["RU 2795 (\"Synapses under Stress\"), Ro2327/13-2","70114276","10.20.2.031MN","VIP+ (03VP10540)","BU 2974/3-2 - SPP2127, EXC-2151-390873048-Cluster of Excellence- ImmunoSensation2","Project-ID 503306912 - FOR5547","SPP2127 GO 2301/5-2","Freigeist-A110720"],"pubmed_authors":["Mariappan A","Lin S","Gabriel E","Muller S","D'Alessandris QG","Freifeld L","Rathinam D","Ramani A","Goureau O","Jadhav V","Gopalakrishnan J","Mirsaidi A","Hadian K","Jurisch-Yaksi N","Altinisik N","Busskamp V","Gerkau NJ","Rose CR","Pasquini G","Vinchure OS","Muotri A","Ricci-Vitiani L","Wollnik B","Windoffer H","Pallini R","Rothenaigner I"],"additional_accession":[]},"is_claimable":false,"name":"Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening.","description":"Brain organoids offer unprecedented insights into brain development and disease modeling and hold promise for drug screening. Significant hindrances, however, are morphological and cellular heterogeneity, inter-organoid size differences, cellular stress, and poor reproducibility. Here, we describe a method that reproducibly generates thousands of organoids across multiple hiPSC lines. These High Quantity brain organoids (Hi-Q brain organoids) exhibit reproducible cytoarchitecture, cell diversity, and functionality, are free from ectopically active cellular stress pathways, and allow cryopreservation and re-culturing. Patient-derived Hi-Q brain organoids recapitulate distinct forms of developmental defects: primary microcephaly due to a mutation in CDK5RAP2 and progeria-associated defects o","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-18T13:01:30.926Z","creation":"2025-04-06T22:27:55.533Z"},"accession":"S-EPMC11659410","cross_references":{"pubmed":["39702477"],"doi":["10.1038/s41467-024-55226-6"]}}