{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Weil PP"],"funding":["Witten/Herdecke University ‘Interne Forschungsförderung’"],"pagination":["30564"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11659419"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Testicular cell differentiation is a highly regulated process, essential for male reproductive health. The histone variant H3.5 is apparently a critical player in this intricate orchestra of cell types, but its regulation and function remains poorly understood. To elucidate its role, we fractionized testicular cells using c-Kit/CD117 as a separation marker and analyzed H3.5 expression. Further, we investigated the regulation of H3.5 expression using public data repositories. We explored DNA methylation patterns in specific regions of the H3-5 gene and assessed H3-5 copy number gain in seminoma specimens. Additionally, we examined the testicular localization of H3.5 and its histone chaperone interactions to understand its regulation at the protein level. We used qRT-PCR, MeDIP, and qPCR to "],"journal":["Scientific reports"],"pubmed_title":["Histone variant H3.5 in testicular cell differentiation and its interactions with histone chaperones."],"pmcid":["PMC11659419"],"funding_grant_id":["IFF2018-56 AND IFF 2023-69"],"pubmed_authors":["Wirth B","Degener S","Oetjen E","Godde D","Cremers JF","Czarnetzki M","Postberg J","Pembaur A","Braun S","Zirngibl K","Weil PP","Busscher H"],"additional_accession":[]},"is_claimable":false,"name":"Histone variant H3.5 in testicular cell differentiation and its interactions with histone chaperones.","description":"Testicular cell differentiation is a highly regulated process, essential for male reproductive health. The histone variant H3.5 is apparently a critical player in this intricate orchestra of cell types, but its regulation and function remains poorly understood. To elucidate its role, we fractionized testicular cells using c-Kit/CD117 as a separation marker and analyzed H3.5 expression. Further, we investigated the regulation of H3.5 expression using public data repositories. We explored DNA methylation patterns in specific regions of the H3-5 gene and assessed H3-5 copy number gain in seminoma specimens. Additionally, we examined the testicular localization of H3.5 and its histone chaperone interactions to understand its regulation at the protein level. We used qRT-PCR, MeDIP, and qPCR to ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T01:09:22.204Z","creation":"2025-04-04T01:09:22.204Z"},"accession":"S-EPMC11659419","cross_references":{"pubmed":["39702777"],"doi":["10.1038/s41598-024-83206-9"]}}