biostudies-literatureUnknown56(11)He X<i>SUN5</i>, a testis-specific gene, is associated with acephalic spermatozoa syndrome (ASS). Here, we demonstrate that SUN5 is involved in mRNA export. In <i>Sun5</i>-knockout mice ( <i>Sun</i>5 ^-/-), poly(A) ⁺ RNA accumulates in the nuclei of germ cells, leading to reduced sperm counts, decreased sperm motility and disrupted sperm head-to-tail junctions. Additionally, in the GC-2 germ cell line with RNA interference of <i>Sun5</i>, heterogeneous nuclear ribonucleoproteins (hnRNPs) and poly (A) ⁺ RNA (mainly mRNA) are retained in the nucleus. Further mechanistic studies reveal that SUN5 interacts with Nxf1 (nuclear RNA export factor 1) and nucleoporin 93 (Nup93). Interference with Nup93 inhibits mRNA export. Treatment with leptomycin B to block the CRM1 paActa biochimica et biophysica Sinica1673-1686https://www.ebi.ac.uk/biostudies/studies/S-EPMC11659784biostudies-literatureSUN5, a testis-specific nuclear membrane protein, participates in recruitment and export of nuclear mRNA in spermatogenesis.PMC11659784Liu XZhang YHe XXing XHuang LSu YMao ZLiu GfalseSUN5, a testis-specific nuclear membrane protein, participates in recruitment and export of nuclear mRNA in spermatogenesis.<i>SUN5</i>, a testis-specific gene, is associated with acephalic spermatozoa syndrome (ASS). Here, we demonstrate that SUN5 is involved in mRNA export. In <i>Sun5</i>-knockout mice ( <i>Sun</i>5 ^-/-), poly(A) ⁺ RNA accumulates in the nuclei of germ cells, leading to reduced sperm counts, decreased sperm motility and disrupted sperm head-to-tail junctions. Additionally, in the GC-2 germ cell line with RNA interference of <i>Sun5</i>, heterogeneous nuclear ribonucleoproteins (hnRNPs) and poly (A) ⁺ RNA (mainly mRNA) are retained in the nucleus. Further mechanistic studies reveal that SUN5 interacts with Nxf1 (nuclear RNA export factor 1) and nucleoporin 93 (Nup93). Interference with Nup93 inhibits mRNA export. Treatment with leptomycin B to block the CRM1 pa2024-01-01T00:00:00Z2024 Aug2026-06-01T19:48:54.288Z2025-04-04T13:41:44.56ZS-EPMC116597843910820710.3724/abbs.2024134