{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shippy TD"],"funding":["U.S. Department of Agriculture","National Institutes of Health","National Institute of Food and Agriculture","NIH HHS","NIGMS NIH HHS"],"pagination":["giae109"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11659978"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<h4>Background</h4>Diaphorina citri is an insect vector of \"Candidatus Liberibacter asiaticus\" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.<h4>Results</h4>To improve the genomic resources needed for targeted pest control, we assembled a D. citri genome based on PacBio long reads followed by proximity ligation-based scaffolding. The 474-Mb genome has 13 chromosomal-length scaffolds. In total, 1,036 genes were manually curated as part of a community annotation project, composed primarily of unde"],"journal":["GigaScience"],"pubmed_title":["Diaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing."],"pmcid":["PMC11659978"],"funding_grant_id":["2015-70016-23028","P20 GM103418","8062-22410-007-000-D","2020-38422-32252","2020-70029-33199","P20GM103418"],"pubmed_authors":["Ventura J","Hunter WB","Neiman A","Hoyt S","Weirauch MT","DeLaFlor Y","Gervais K","Perentesis KMR","Wise T","Panitz N","Hosmani PS","Davis N","Thate A","Shippy TD","Masse R","Holcomb R","Chen X","de Oliveira L","Brown SJ","Vosberg C","Tank W","Grace R","Shatters RG","Kennedy B","Benoit JB","Heck M","Mathew A","Chen C","Lu A","Paris T","Adkins S","Vandervoort S","Flores-Gonzalez M","Kemp M","Tamayo B","Massimino C","Bailey ST","Espino M","Sena MM","Miller S","Mann M","Ortiz Y","Norman S","Harper D","Licata S","Kruse A","Michels E","Jernigan MR","Klaessan S","Michels S","Reynolds M","Mueller LA","Weis N","D'Elia T","Saha S","Norus J","Adams R","Hoang M","Hasan DL","Kercher K","Perry M"],"additional_accession":[]},"is_claimable":false,"name":"Diaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.","description":"<h4>Background</h4>Diaphorina citri is an insect vector of \"Candidatus Liberibacter asiaticus\" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.<h4>Results</h4>To improve the genomic resources needed for targeted pest control, we assembled a D. citri genome based on PacBio long reads followed by proximity ligation-based scaffolding. The 474-Mb genome has 13 chromosomal-length scaffolds. In total, 1,036 genes were manually curated as part of a community annotation project, composed primarily of unde","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-06-03T00:47:21.407Z","creation":"2025-04-04T00:38:24.163Z"},"accession":"S-EPMC11659978","cross_references":{"pubmed":["39704701"],"doi":["10.1093/gigascience/giae109"]}}