biostudies-literatureShippy TDU.S. Department of AgricultureNational Institutes of HealthNational Institute of Food and AgricultureNIH HHSNIGMS NIH HHSgiae109https://www.ebi.ac.uk/biostudies/studies/S-EPMC11659978biostudies-literatureUnknown13<h4>Background</h4>Diaphorina citri is an insect vector of "Candidatus Liberibacter asiaticus" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.<h4>Results</h4>To improve the genomic resources needed for targeted pest control, we assembled a D. citri genome based on PacBio long reads followed by proximity ligation-based scaffolding. The 474-Mb genome has 13 chromosomal-length scaffolds. In total, 1,036 genes were manually curated as part of a community annotation project, composed primarily of undeGigaScienceDiaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.PMC116599782015-70016-23028P20 GM1034188062-22410-007-000-D2020-38422-322522020-70029-33199P20GM103418Ventura JHunter WBNeiman AHoyt SWeirauch MTDeLaFlor YGervais KPerentesis KMRWise TPanitz NHosmani PSDavis NThate AShippy TDMasse RHolcomb RChen Xde Oliveira LBrown SJVosberg CTank WGrace RShatters RGKennedy BBenoit JBHeck MMathew AChen CLu AParis TAdkins SVandervoort SFlores-Gonzalez MKemp MTamayo BMassimino CBailey STEspino MSena MMMiller SMann MOrtiz YNorman SHarper DLicata SKruse AMichels EJernigan MRKlaessan SMichels SReynolds MMueller LAWeis ND'Elia TSaha SNorus JAdams RHoang MHasan DLKercher KPerry MfalseDiaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.<h4>Background</h4>Diaphorina citri is an insect vector of "Candidatus Liberibacter asiaticus" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.<h4>Results</h4>To improve the genomic resources needed for targeted pest control, we assembled a D. citri genome based on PacBio long reads followed by proximity ligation-based scaffolding. The 474-Mb genome has 13 chromosomal-length scaffolds. In total, 1,036 genes were manually curated as part of a community annotation project, composed primarily of unde2024-01-01T00:00:00Z2024 Jan2026-06-03T00:47:21.407Z2025-04-04T00:38:24.163ZS-EPMC116599783970470110.1093/gigascience/giae109