biostudies-literatureChen WSanya Science and Technology Innovation Special ProjectNational Natural Science Foundation of ChinaGeneral Program of Natural Science Foundation of Hainan ProvinceKey Research and Development Program in Hainan Province368-380https://www.ebi.ac.uk/biostudies/studies/S-EPMC11662979biostudies-literatureUnknown73(2)Efficient clearance of hematomas is crucial for improving clinical outcomes in patients with intracerebral hemorrhage (ICH). The glymphatic system, facilitated by aquaporin-4 (AQP4), plays a crucial role in cerebrospinal fluid (CSF) entry and metabolic waste clearance. This study examined the role of the glymphatic system in ICH pathology, with a focus on AQP4. Collagenase-induced ICH models were established, with AQP4 expression regulated through mifepristone as an agonist, TGN-020 as an inhibitor, and Aqp4 gene knockout. Fluorescence tracing and multimodal magnetic resonance imaging (MRI) were employed to observe glymphatic system functionality, hematoma, and edema volumes. Neurological deficit scoring was performed using the modified Garcia Scale. AQP4 expression was quantified using RTGliaAquaporin-4 activation facilitates glymphatic system function and hematoma clearance post-intracerebral hemorrhage.PMC116629792022KJCX248216023ZDYF2023 SHFZ104822MS210Liang CXue FLiu YPeng SLian XChen WWang GBao SfalseAquaporin-4 activation facilitates glymphatic system function and hematoma clearance post-intracerebral hemorrhage.Efficient clearance of hematomas is crucial for improving clinical outcomes in patients with intracerebral hemorrhage (ICH). The glymphatic system, facilitated by aquaporin-4 (AQP4), plays a crucial role in cerebrospinal fluid (CSF) entry and metabolic waste clearance. This study examined the role of the glymphatic system in ICH pathology, with a focus on AQP4. Collagenase-induced ICH models were established, with AQP4 expression regulated through mifepristone as an agonist, TGN-020 as an inhibitor, and Aqp4 gene knockout. Fluorescence tracing and multimodal magnetic resonance imaging (MRI) were employed to observe glymphatic system functionality, hematoma, and edema volumes. Neurological deficit scoring was performed using the modified Garcia Scale. AQP4 expression was quantified using RT2025-01-01T00:00:00Z2025 Feb2025-04-18T14:20:35.197Z2025-04-07T00:29:27.699ZS-EPMC116629793953019610.1002/glia.24639