biostudies-literatureUnknown13(1)Neutel CHG<h4>Introduction</h4>Arterial stiffening is a hallmark of vascular ageing, and unravelling its underlying mechanisms has become a central theme in the field of cardiovascular disease. While various techniques and experimental setups are accessible for investigating biomechanics of blood vessels both in vivo and ex vivo, comparing findings across diverse methodologies is challenging.<h4>Methods</h4>Arterial stiffness in the aorta of adult (5 months) and aged (24 months) wild-type C57Bl/6J mice was measured in vivo, after which ex vivo biomechanical evaluation was performed using the Rodent Oscillatory Tension Setup to study Arterial Compliance (ROTSAC; University of Antwerp, Belgium) and the DynamX setup (Maastricht University, The Netherlands). Stiffness of aortic tissue was measured in boPulse (Basel, Switzerland)1-10https://www.ebi.ac.uk/biostudies/studies/S-EPMC11666261biostudies-literatureThe MAASWERP Study: An International, Comparative Case Study on Measuring Biomechanics of the Aged Murine Aorta.PMC11666261Reesink KDGuns PJNeutel CHGSchalkwijk CGMartinet WDelhaas Tvan der Laan KWFWesley CDPencheva MGGiudici AKruger DNDe Meyer GRYSpronck BfalseThe MAASWERP Study: An International, Comparative Case Study on Measuring Biomechanics of the Aged Murine Aorta.<h4>Introduction</h4>Arterial stiffening is a hallmark of vascular ageing, and unravelling its underlying mechanisms has become a central theme in the field of cardiovascular disease. While various techniques and experimental setups are accessible for investigating biomechanics of blood vessels both in vivo and ex vivo, comparing findings across diverse methodologies is challenging.<h4>Methods</h4>Arterial stiffness in the aorta of adult (5 months) and aged (24 months) wild-type C57Bl/6J mice was measured in vivo, after which ex vivo biomechanical evaluation was performed using the Rodent Oscillatory Tension Setup to study Arterial Compliance (ROTSAC; University of Antwerp, Belgium) and the DynamX setup (Maastricht University, The Netherlands). Stiffness of aortic tissue was measured in bo2025-01-01T00:00:00Z2025 Jan-Dec2026-06-06T08:47:53.236Z2026-05-28T03:10:58.797ZS-EPMC116662613972010110.1159/000542694